Articles
POTATO CRYOPRESERVATION IN GERMANY – USING THE DROPLET METHOD FOR THE ESTABLISHMENT OF A NEW LARGE COLLECTION
Article number
623_20
Pages
193 – 200
Language
English
Abstract
Potato germplasm has been stored in cryopreservation since 1997. The droplet method is applied using 10% dimethyl sulfoxide as cryoprotectant.
Explants are fixed at pieces of aluminium foil and frozen ultra-rapidly.
Regeneration rates depend on the genotype and range from 20 to 100% per accession.
The average survival rate is 72%; the mean regeneration is 48%. The explants are obtained from a collection based on in vitro cycles including storage of microtubers.
Donor plantlets are grown from microtubers on MS medium without hormones.
Once they have reached lengths of 5 cm, apical explants are dissected for cryopreservation.
A comparison has been made with plantlets grown as shoot cultures for several years.
Microtuber-derived plantlets gave significantly better results.
To further improve the regeneration rates, experiments have been performed using different culture vessel types.
Explants are fixed at pieces of aluminium foil and frozen ultra-rapidly.
Regeneration rates depend on the genotype and range from 20 to 100% per accession.
The average survival rate is 72%; the mean regeneration is 48%. The explants are obtained from a collection based on in vitro cycles including storage of microtubers.
Donor plantlets are grown from microtubers on MS medium without hormones.
Once they have reached lengths of 5 cm, apical explants are dissected for cryopreservation.
A comparison has been made with plantlets grown as shoot cultures for several years.
Microtuber-derived plantlets gave significantly better results.
To further improve the regeneration rates, experiments have been performed using different culture vessel types.
Publication
Authors
E.R.J. Keller, M. Dreiling
Keywords
Solanum tuberosum, culture vessels, in vitro genebanking, regrowth rates
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