Home > XXVI International Horticultural Congress: Biotechnology in Horticultural Crop Improvement: Achievements, Opportunities and Limitations > MICROPROPAGATION OF CHOCOLATE COSMOS (COSMOS ATROSANGUINEUS) BY REPEATED DIVISION OF NODES/AXILLARY SHOOTS AND ADVENTITIOUS SHOOTS FROM MICROSHOOTS

Articles

MICROPROPAGATION OF CHOCOLATE COSMOS (COSMOS ATROSANGUINEUS) BY REPEATED DIVISION OF NODES/AXILLARY SHOOTS AND ADVENTITIOUS SHOOTS FROM MICROSHOOTS

Article number

625_30

Pages

261 – 264

Language

English

Abstract

Micropropagation of chocolate cosmos was achieved by subculturing stem section with nodes or dividing axillary shoots on MS (Murashige and Skoog, 1962)- based medium with benzyladenine (BA). The optimal multiplication rate of 3.7 per 17- day culture period was achieved without vitrification on medium containing 0.88 μM BA. Shoots elongated from in vitro cultured microcuttings rooted (60%) on medium with 4.9μM indole-3-butyric acid (IBA). Most plantlets (93%) were acclimatized ex vitro.
For the second experiment, plant regeneration from the cultured tissue was attempted.
Stem, internode, leaf or petiole with leaf was cut into sections and cultured on the medium with 0, 0.19 or 1.9 μM alpha -naphtaleneacetic acid (NAA) combined with 4.4 or 18 μM benzyladenin (BA). The highest regeneration rate (80%) and shoot number (5.3) per internode section were obtained on the medium with 4.4 μM BA alone.
Supplement with 1.9 μM NAA completely suppressed shoot regeneration regardless of BA concentration.

Publication

XXVI International Horticultural Congress: Biotechnology in Horticultural Crop Improvement: Achievements, Opportunities and Limitations

Authors

T. Hosoki, H. Kobayakawa, K. Ohta

Keywords

micropropagation, Cosmos atrosanguineus, tissue culture, adventitious bud formation

Full text

https://doi.org/10.17660/ActaHortic.2003.625.30

Online Articles (56)

2. NAVIGATING THE ‘SMÖRGÅSBORD’ OF DNA MARKER METHODS FOR IMPROVEMENT OF HORTICULTURAL CROPS

H. Nybom

3. PROGRESS IN APPLICATION OF MOLECULAR MARKERS TO GENETIC IMPROVEMENT OF HORTICULTURAL CROPS

S. Rajapakse

4. PLANT CULTIVAR IDENTIFICATION AND REGISTRATION – THE ROLE FOR MOLECULAR TECHNIQUES

M.S. Camlin

5. SEQUENCE COMPARISON AND CHARACTERIZATION OF DNA FRAGMENTS AMPLIFIED BY RESISTANCE GENE PRIMERS IN TOMATO

D.O. Niño-Liu | L.P. Zhang | M.R. Foolad

6. IDENTIFICATION OF MOLECULAR MARKERS ASSOCIATED WITH COLD TOLERANCE IN BLUEBERRY

L.J. Rowland | S. Mehra | R. Arora

7. ANALYSIS OF ORGAN-SPECIFIC AND -PREFERENTIALLY EXPRESSED GENES FROM ANTHER AND TEPAL OF LILY

H.-J. Yu | E.-J. Suh | B.W. Yae | B.-H. Han | I.-G. Mok

8. POSITIONAL CLONING OF THE APPLE SCAB RESISTANCE GENE VF

M. Xu | S.S. Korban

9. DEVELOPMENT OF RAPD AND SCAR MARKERS LINKED TO FLESH ADHESION GENE IN PEACH

J.H. Jun | K.H. Chung | S.B. Jeong | H.J. Lee

10. RAPD MARKERS LINKED TO THE DISEASE RESISTANCE GENES IN CHINESE WILD VITIS

W. Yuejin | Z. Jianxia | W. Xiping | X. Yan | W. Yizhen

11. DEVELOPMENT OF RAPD-AFLP MAP OF EGGPLANT AND IMPROVEMENT OF TOLERANCE TO VERTICILLIUM WILT

F. Sunseri | A. Sciancalepore | G. Martelli | N. Acciarri | G.L. Rotino | D. Valentino | G. Tamietti

12. MOLECULAR CHARACTERIZATION OF RIPENING FRUIT PROCESSES IN STRAWBERRY STARTING FROM A TRANSCRIBED GENOMIC DNA FRACTION

G. Martelli | M.R. Sabina | A. Sciancalepore | F. Sunseri | I. Greco

13. MOLECULAR MECHANISM OF GRAFT TRANSFORMATION IN CAPSICUM ANNUUM

Y. Hirata | S. Ogata | S. Kurita | G.T. Nozawa | J. Zhou | S. Wu

14. CONSTRUCTION OF A LYCHEE GENETIC LINKAGE MAP BASED ON RAPD MARKERS

C. Liu | M. Mei

15. CYCLAMEN PERSICUM MILL.: SOMATIC EMBRYOGENESIS AND RAPD ANALYSIS OF EMBRYOGENIC CALLUS

M. Laura | L. De Benedetti | .S Bruna | G. Burchi | T. Berio | A. Giovannini | B. Ruffoni

16. HIGH FREQUENCY RECOVERY OF INTERGENERIC FUSION PRODUCTS OF BRASSICA OLERACEA (+) LEPIDIUM MEYENII AND THEIR MOLECULAR CHARACTERIZATION BY RAPD AND AFLP

U. Ryschka | E. Klocke | G. Schumann

17. STRESS AND QUALITY IN IN VITRO CULTURE

A.C. Cassells | S.M. Joyce | E.A. O'Herlihy | M.J. Perez-Sanz | C. Walsh

18. BIOTIZATION OF MICROPLANTS FOR IMPROVED PERFORMANCE

S. Gianinazzi | L. Oubaha | M. Chahbandar | B. Blal | M.-C. Lemoine

19. LIQUID CULTURE SYSTEMS FOR PLANT PROPAGATION

A.K.H. Eide | C. Munster | P.H. Heyerdahl | R. Lyngved | O.A.S. Olsen

20. APPLICATION OF BIOREACTOR CULTURE FOR LARGE SCALE PRODUCTION OF CHRYSANTHEMUM TRANSPLANTS

S.J. Kim | E.J. Hahn | K.Y. Paek | H.N. Murthy

21. TEMPERATE FRUIT PLANT PROPAGATION THROUGH TEMPORARY IMMERSION

C. Damiano | S. Monticelli | S.R. La Starza | A. Gentile | A. Frattarelli

22. AUXIN, CALCIUM AND SODIUM IN SOMATIC EMBRYOGENESIS OF AFRICAN VIOLET (SAINTPAULIA IONANTHA WENDL. CV. BENJAMIN)

S.J. Murch | J.M.R. Victor | P.K. Saxena

23. IN VITRO “REJUVENATION” OF WOODY SPECIES IS TEMPORARY

M.N. Nas | P.E. Read | V. Miller | P.A. Rutter

24. SOMATIC EMBRYOGENESIS OF LETTUCE FROM MATURE TISSUES

J.E.A. Seabrook | L.K. Douglass

25. PRODUCTION OF SOMATIC EMBRYOS OF AMERICAN GINSENG IN SUSPENSION CULTURE AND REGENERATION OF PLANTLETS

M. Feeney | Z.K. Punja

26. THE INFLUENCE OF THIDIAZURON ON IN VITRO SHOOT PROLIFERATION OF OAKLEAF HYDRANGEA (HYDRANGEA QUERCIFOLIA BARTR.)

J.E. Preece | D.I. Ledbetter

27. DIFFERENTIATION SPECIFIC GENE EXPRESSION IN REGAL (LILIUM REGALE) CALLUS

V.-P. Pelkonen | A. Hohtola | H. Häggman | K. Laine

28. CLONAL PROPAGATION OF HYBRID SWEETGUM (LIQUIDAMBAR STYRACIFLUA x L. FORMOSANA) VIA SOMATIC EMBRYOGENESIS FOR ORNAMENTAL APPLICATION

W. Vendrame | C.P. Holliday | S.A. Merkle

29. THE INFLUENCE OF SOURCE TREE ON SOMATIC EMBRYOGENESIS FROM EASTERN BLACK WALNUT (JUGLANS NIGRA) IMMATURE COTYLEDONS

M.M. Steger | J.E. Preece

30. UTILIZATION OF EMBRYOGENIC CELL CULTURES FOR THE MASS PRODUCTION OF BULBLETS IN ORIENTAL AND EASTER LILIES

S.K. Kim | J.S. Lee | K.H. Huang | B.J. Ahn

31. MICROPROPAGATION OF CHOCOLATE COSMOS (COSMOS ATROSANGUINEUS) BY REPEATED DIVISION OF NODES/AXILLARY SHOOTS AND ADVENTITIOUS SHOOTS FROM MICROSHOOTS

T. Hosoki | H. Kobayakawa | K. Ohta

32. RAPID MULTIPLICATION OF BASIL (OCIMUM BASILICUM); FACTORS AFFECTING CALLUS FORMATION AND PLANT REGENERATION

K.-J. Lee | B.-Y. Yi

33. PHOTOAUTOTROPHIC CULTURE WITH CO₂ ENRICHMENT FOR IMPROVING MICROPROPAGATION OF COFFEA ARABUSTA USING SOMATIC EMBRYOS

A. Uno | K. Ohyama | T. Kozai | C. Kubota

34. STUDIES ON THE APPLYING OF EMBRYO CULTURE IN BREEDING NEW HYBRIDS BY CROSSING SEEDLESS GRAPE CULTIVARS

N. Kebeli | Y. Boz | C. Özer

35. MICROPROPAGATION OF JUVENILE EUCALYPTUS CITRIODORA

E.M. Koriesh | Y.M. Abd El-Fattah | M.A. El-Dayem | M.A. El-Etriby

36. METHYL JASMONATE INCREASES SAPONIN CONTENT IN BIOREACTOR CULTURE OF GINSENG (PANAX GINSENG C.A. MEYER) ADVENTITIOUS ROOTS

Y.-S. Kim | D. Chakrabarty | E.-J. Hahn | K.-Y. Paek

37. TECHNOLOGIES FOR MANIPULATING QUALITY AND PRODUCTIVITY TRAITS IN HORTICULTURAL CROPS

A.M. Dandekar

38. TECHNOLOGIES FOR IMPROVING TOLERANCE TO ENVIRONMENTAL STRESS: GENETIC ENGINEERING OF SALT STRESS TOLERANCE IN JAPANESE PERSIMMON (DIOSPYROS KAKI) WITH THE GENES INVOLVED IN BIOSYNTHESIS OF COMPATIBLE SOLUTE

R. Tao | M. Gao

39. TARGETING TRANSGENE EXPRESSION IN APPLE TREE TISSUES -ROLES FOR HETEROLOGOUS AND HOMOLOGOUS PROMOTERS

D.J. James | A.J. Massiah | E.R. Hiles | J.R. Gittins | S. Biricolti | T.K. Pellny

40. DETECTION OF TRANSGENES IN APPLE ROOTSTOCKS USING ANCHORED PCR

L.H. Zhu | M. Welander | P. Windels | M. De Loose

41. THE BREAK -THROUGH IN THE REDUCTION OF JUVENILE PHASE IN APPLE USING TRANSGENIC APPROACHES

N. Kotoda | M. Wada | T. Masuda | J. Soejima

42. DNA METHYLATION VS. SOMACLONAL VARIATION IN HIGHER PLANTS: OIL PALM AS A CASE STUDY

E. Jaligot | T. Beulé | J.-L. Verdeil | J. Tregear | A. Rival

43. TRANSFORMATION OF POTATO PLANTS WITH THE ISOPENTENYLTRANSFERASE GENE LEADS TO ENHANCED STOLON DEVELOPMENT AND PRECOCIOUS TUBER SPROUTING

A. Lafta | J. Wu | J. Lorenzen

44. PRE-CONDITIONING RHODODENDRON AND CHRYSANTHEMUM LEAF EXPLANTS AFFECTS TRANSIENT BETA-GLUCURONIDASE EXPRESSION AFTER BIOLISTIC PARTICLE BOMBARDMENT

C.R. Moore | R.R. Tripepi

45. LEAF SENESCENCE IN PUTATIVE TRANSGENIC PETUNIA PLANTS CONTAINING THE P_SAG12 –KN1 GENE

F.L. Cuquel | K. Minami | D.G. Clark

46. PHENOTYPIC CHARACTERIZATION OF PETUNIA PLANTS EXPRESSING AN INDOLEACETIC ACID (IAA)-LYSINE SYNTHETASE TRANSGENE DRIVEN BY A SHOOT SPECIFIC PROMOTER

R. McAvoy | M. Khodakovskaya | Y. Li | Y. Wu | S. Xue

47. TRANSFORMATION OF PEAR (PYRUS COMMUNIS CV. ‘LA FRANCE’) WITH GENES INVOLVED IN ETHYLENE BIOSYNTHESIS

H. Murayama | T. Toyomasu | W. Mitsuhashi | A.M. Dandekar | M. Gao | N. Matsuta | K. Nishimura | R. Tao

48. TISSUE CULTURE OF AMERICAN GINGSENG AND GENETIC ENGINEERING TO EXPRESS ANTIFUNGAL PROTEINS

Z.K. Punja | W.P. Chen

49. PLANT REGENERATION FROM LEAF EXPLANTS AND EFFICIENT AGROBACTERIUM-MEDIATED TRANSFORMATION SYSTEM OF CHRYSANTHEMUM (DENDRANTHEMA GRANDIFLORUM)

S.Y. Seo | D.C. Choi | J.M. Kim | H.C. Lim | H.J. Kim | J.S. Choi | Y.G. Choi

50. PRELIMINARY RESULTS ON THE EFFECT OF MAGNETIC FIELDS ON ANTHER CULTURE AND POLLEN GERMINATION OF CITRUS CLEMENTINA HORT. EX TAN

M.A. Germanà | B. Chiancone | M.R. Melati | A. Firetto

51. NEW BREEDING METHODS IN SWEDE -PURE LINE VARIETIES SELECTED FROM MICROSPORE-DERIVED LINES

M. Hansen | E. Bratberg

52. PLANT GENETIC ENGINEERING IS A FACT, IT IS AN EFFICIENT AND NECESSARY BREEDING TECHNIQUE, AND IT IS BOTH A CHALLENGE AND A SOLUTION THAT WILL BE ACCEPTED BY THE PUBLIC

A. Altman

53. THE CONGESTED INTELLECTUAL PROPERTY LANDSCAPE AND ITS EFFECT ON THE USE OF BIOTECHNOLOGY FOR THE DEVELOPMENT OF HORTICULTURAL AND SUBSISTENCE CROPS

D. Delmer

54. PUBLIC PERCEPTIONS OF AGRICULTURAL BIOTECHNOLOGY IN THE UNITED STATES IN 2001

W.K. Hallman | A.O. Adelaja | B.J. Schilling

55. EXPRESSION OF CRY1AC AND GUS IN CABBAGE AND CAULIFLOWER

H. Wang | E. Tsang | J. McNeil | C. Hannam | D.C.W. Brown | B. Miki

56. PHOSPHINOTHRICIN- AND CROWN GALL-RESISTANT TRANSGENIC PLANTS OF GRAPEVINE

M.A. Rubtsova | B.A. Levenko