STUDIES ON THE APPLYING OF EMBRYO CULTURE IN BREEDING NEW HYBRIDS BY CROSSING SEEDLESS GRAPE CULTIVARS

This study was carried out to search the possibilities of propagating plants from ovules and embryos of seedless x seedless table grape crosses.
Ovules of 3A/261x Barış and 15/A-61x Barış crosses cultured 42 and 52 days after artificial pollination.
E20A and ER + WP medias were used in culturing of ovules and embryos.
It had been observed that there was no difference between the periods of time in according to viable embryo number for two crosses in E20A media.
In ER media, only 3/A-261x Barış ovules which had been taken 52 days after pollination gave higher viable embryo number than 42 days.
The germination rate of viable embryos of 3/A-261x Barış combination was at least in the 42^th day + ER treatment.
These ratios were 31.52% in ER media and 80.21% in E20A media for ovules of 15/A-61 x Barış that had been cultured in 52^th day.
The treatment of 52^th day + E20A media gave the highest number (21.77%) of seedlings through ovules of 15/A-61x Barış combination.
This number was lower in both two periods and two medias for 3/A-261x Barış ovules.