DEVELOPMENT OF RAPD AND SCAR MARKERS LINKED TO FLESH ADHESION GENE IN PEACH

Flesh adhesion to the stone in peach (Prunus persica [L.] Batsch) fruits can be categorized into three groups of freestone, semi-clingstone and clingstone.
The flesh adhesion is known to be determined by a single gene pair F/f with freestone dominant to clingstone.
Bulked segregant analysis and random amplified polymorphic DNA (RAPD) analysis were performed to detect the markers linked to the F/f gene using F₁ progenies of the clingstone ‘Yumyeong’ (ff) ´ freestone ‘Baekhyang’ (Ff). Four dominant coupling-phase RAPD markers linked to the gene, designated as OPB05₁₃₀₀, OPI07₁₀₅₀, UBC439₁₄₀₀ and OPD20₅₈₀, were identified from 578 10-base primers examined.
The co-segregation of the four markers and the F/f gene was verified using the 95 individuals of ‘Yumyeong’ ´ ‘Baekhyang’. The pooled recombinant frequencies were estimated and converted into genetic distances and linkage group order scores.
The resulting linkage map spanned 16.2 cM and comprised three markers located on the same side of the F/f gene and one marker located on the other side.
Two RAPD markers UBC439₁₄₀₀ and OPB05₁₃₀₀ were developed into dominant sequence characterized amplified region (SCAR) markers.
The SCAR markers were found to be adequate to identify the F/f gene in segregating progenies and commercial cultivars.
These markers could reliably be used in the marker-assisted selection of peach germplasm at an early developmental stage of the trees.

XXVI International Horticultural Congress: Biotechnology in Horticultural Crop Improvement: Achievements, Opportunities and Limitations

J.H. Jun, K.H. Chung, S.B. Jeong, H.J. Lee

Bulked segregant analysis, Clingstone, Freestone, Genetic map, Marker-assisted selection, Molecular markers

https://doi.org/10.17660/ActaHortic.2003.625.8