Articles
DEVELOPMENT OF AN IN VITRO WEANING SYSTEM FOR GARRYA X ISSAQUAHENSIS NELSON ‘GLASNEVIN WINE’
Article number
630_31
Pages
255 – 261
Language
English
Abstract
Glasnevin Wine is a hybrid of commercial value to the hardy nursery stock industry, prized for its extensive catkins.
The hybrid can be micropropagated relatively easily but ex vitro establishment has proven very difficult.
An alternative system was therefore developed in which the rooting plants were covered with a range of transparent plastic membranes with varying porosities. Under the most porous membrane, scanning electron micrographs of the developing leaves showed stomata in a wide range of positions from fully opened to fully closed.
Leaves forming under other membranes showed all stomata remaining open.
There was a direct correlation between water loss and membrane cover which matched the membrane classification.
Plants that had been weaned under membranes with a water vapour transmission rate (wvtr – (g/m2/24h) greater than 300 established well ex vitro.
Plants weaned at between 200-300 (wvtr) established poorly while those weaned below 200 (wvtr) died.
The density and size of stomata decreased under membranes.
Currently the membrane with a water vapour transmission rate of 422 is being used to establish the plants ex vitro.
The hybrid can be micropropagated relatively easily but ex vitro establishment has proven very difficult.
An alternative system was therefore developed in which the rooting plants were covered with a range of transparent plastic membranes with varying porosities. Under the most porous membrane, scanning electron micrographs of the developing leaves showed stomata in a wide range of positions from fully opened to fully closed.
Leaves forming under other membranes showed all stomata remaining open.
There was a direct correlation between water loss and membrane cover which matched the membrane classification.
Plants that had been weaned under membranes with a water vapour transmission rate (wvtr – (g/m2/24h) greater than 300 established well ex vitro.
Plants weaned at between 200-300 (wvtr) established poorly while those weaned below 200 (wvtr) died.
The density and size of stomata decreased under membranes.
Currently the membrane with a water vapour transmission rate of 422 is being used to establish the plants ex vitro.
Publication
Authors
S. Mac An tSaoir, K. Lappin, D. McCall
Keywords
diffusion resistance, ex vitro establishment, plant tissue culture, stomata
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