EFFECT OF CARBON DIOXIDE, NUTRIENT COMPOSITION AND SUPPORTING MATERIAL ON THE GROWTH OF MINIATURE ROSE ‘SILK RED’ IN VITRO

Growth and development of miniature rose ‘Silk Red’ plantlets in vitro as affected by various environmental factors were evaluated.
Treatments used were two levels each of trophic phase (photoautotrophic and photomixotrophic) and nutrient composition (half strength MS basal solution and Gato hydroponic rose solution), and three supporting materials (agar, gelrite, and Tosilee medium, a commercial plug medium). Photomixotrophically micropropagated plantlets excised of roots were used as explants.
Explants were cultured for four weeks under cool-white fluorescent lamps at a light intensity of 180 μmol-m^-2-s^-1 for 16 h-day^-1, at 25°C±1 temperature, and 70-80 % relative humidity.
Height, numbers of leaves, branches and flowers, length of the longest leaf, chlorophyll concentration, numbers of main and fine roots, and length of the longest root were promoted with increased CO₂ concentration.
Plantlet growth under a photoautotrophic culture condition was enhanced in the Tosilee medium supplemented with Gato hydroponic solution as compared to agar or gelrite.

XXVI International Horticultural Congress: Nursery Crops; Development, Evaluation, Production and Use

G. Hee Kim, E. Young Nam, B. Ryong Jeong

photoautotrophic culture, nutrient solution, micropropagation, growing medium, multiple shoots

https://doi.org/10.17660/ActaHortic.2004.630.33