Articles
RAPD-PCR ANSWERS SOME LONG-STANDING QUESTIONS ABOUT ROSE IDENTIFICATION
Article number
634_10
Pages
85 – 89
Language
English
Abstract
In the past 20 years, there has been a resurgence of interest in historic, heritage, or antique rose cultivars.
Numerous commercial nurseries now specialize in producing old roses.
In many cases, there is little or no evidence that the rose currently being grown is the cultivar historically connected with the name being used.
In some cases, a rose sold under a historic name by one nursery may not be the same cultivar as is being sold under the same name by a different nursery.
Also, historic records of roses parentage are not always accurate.
This paper illustrates how DNA analysis using randomly amplified polymorphic DNA (RAPD) analysis via the polymerase chain reaction (PCR) can assist in answering such questions about the identity and relationships of old roses.
The early members of the Noisette class were studied – the parentage of Champneys Pink Cluster, the relationship of that rose to Blush Noisette, and the relationship of the various forms of Rosa moschata currently in commerce.
Also, accessions of Champneys Pink Cluster and Blush Noisette from multiple nurseries were compared, to determine if they are all the same clones.
Numerous commercial nurseries now specialize in producing old roses.
In many cases, there is little or no evidence that the rose currently being grown is the cultivar historically connected with the name being used.
In some cases, a rose sold under a historic name by one nursery may not be the same cultivar as is being sold under the same name by a different nursery.
Also, historic records of roses parentage are not always accurate.
This paper illustrates how DNA analysis using randomly amplified polymorphic DNA (RAPD) analysis via the polymerase chain reaction (PCR) can assist in answering such questions about the identity and relationships of old roses.
The early members of the Noisette class were studied – the parentage of Champneys Pink Cluster, the relationship of that rose to Blush Noisette, and the relationship of the various forms of Rosa moschata currently in commerce.
Also, accessions of Champneys Pink Cluster and Blush Noisette from multiple nurseries were compared, to determine if they are all the same clones.
Publication
Authors
M.M. Manners, N. Morvillo, C. Frederick, A. Wagner
Keywords
Rosa moschata, Noisette, DNA analysis
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