Articles
AFLP, ISSR AND SSR POLYMORPHISMS ARE IN ACCORDANCE WITH BOTANICAL AND CULTIVATED PLANT TAXONOMIES OF THE HIGHLY POLYMORPHIC CUCURBITA PEPO
Article number
634_20
Pages
167 – 173
Language
English
Abstract
Cucurbita pepo (pumpkin, squash, gourd) is extremely variable in fruit characteristics.
According to recent botanical and cultivated-plant taxonomical treatments that were based on variations in allozymes and fruit shape, C. pepo consists of three subspecies containing wild and cultivated, inedible, small-fruited sorts (gourds) and eight groups of edible, large-fruited cultivars (pumpkins and squash). Our objective was to determine if these treatments reflect genetic relationships as viewed at the DNA level, through the use AFLP, ISSR, and SSR markers.
Forty-five accessions were compared for presence or absence of 448 AFLP, 147 ISSR, and 20 SSR bands, their genetic distances (GDs) were estimated, and UPGMA cluster analysis was conducted.
Correlation coefficients were 0.95 between AFLPs and ISSRs, 0.78 between AFLPs and SSRs, and 0.77 between ISSRs and SSRs, all three comparisons with P << 0.001. Overall, clustering and sub-clustering were much in accordance with two highly polygenic characteristics, fruit shape and size.
Clustering occurred in accordance with subspecies and sub-clustering in accordance with cultivar-groups.
Within-group GDs were less than corresponding between-group GDs in nearly all comparisons.
The smallest-fruited accession, Miniature Ball, occupied a central position within C. pepo.
According to recent botanical and cultivated-plant taxonomical treatments that were based on variations in allozymes and fruit shape, C. pepo consists of three subspecies containing wild and cultivated, inedible, small-fruited sorts (gourds) and eight groups of edible, large-fruited cultivars (pumpkins and squash). Our objective was to determine if these treatments reflect genetic relationships as viewed at the DNA level, through the use AFLP, ISSR, and SSR markers.
Forty-five accessions were compared for presence or absence of 448 AFLP, 147 ISSR, and 20 SSR bands, their genetic distances (GDs) were estimated, and UPGMA cluster analysis was conducted.
Correlation coefficients were 0.95 between AFLPs and ISSRs, 0.78 between AFLPs and SSRs, and 0.77 between ISSRs and SSRs, all three comparisons with P << 0.001. Overall, clustering and sub-clustering were much in accordance with two highly polygenic characteristics, fruit shape and size.
Clustering occurred in accordance with subspecies and sub-clustering in accordance with cultivar-groups.
Within-group GDs were less than corresponding between-group GDs in nearly all comparisons.
The smallest-fruited accession, Miniature Ball, occupied a central position within C. pepo.
Publication
Authors
H.S. Paris, V. Portnoy, N. Mozes-Daube, G. Tzuri, N. Katzir, N. Yonash
Keywords
pumpkin, squash, gourd, diversity, subspecies, cultivar-groups
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