Home > XI Eucarpia Symposium on Fruit Breeding and Genetics > EFFICIENCY OF RAPD MARKERS FOR VARIETAL IDENTIFICATION IN OLIVE (OLEA EUROPAEA L.) GERMPLASM COLLECTIONS

Articles

EFFICIENCY OF RAPD MARKERS FOR VARIETAL IDENTIFICATION IN OLIVE (OLEA EUROPAEA L.) GERMPLASM COLLECTIONS

Article number

663_115

Pages

643 – 646

Language

English

Abstract

The aim of this study was to evaluate the resolving power of RAPD markers for rapid and reliable identification of olive cultivars in germplasm collections.
The D parameter (the probability that two randomly chosen cultivars have different banding patterns), used for that purpose, showed high values for most of the 21 primers used and its values ranged from 0.6114 (OPI-13) to 0.9762 (OPK-16) with a mean value of 0.8566. This parameter was used to select the five most discriminating primers: OPK-16, OPA-19, OPX-09, OPF-06 and OPZ-11. The joint confusion probability and the statistical number of undistinguishable pairs of cultivars were estimated for these primers (under independence hypothesis). The combination of three primers (OPK-16, OPA-19 and OPX-09) was found optimal for rapid discrimination of 103 cultivars with a very low value of cumulative confusion probability (1.72 x 10^-5), leaving 0.0903 pairs of cultivars undistinguishable.
This fact together with the efficiency of the most discriminating primers combination on an increasing number of cultivars proved the utility of RAPD markers for varietal identification of olive cultivars in collections and in nurseries.

Publication

XI Eucarpia Symposium on Fruit Breeding and Genetics

Authors

A. Belaj, L. Rallo, I. Trujillo, Z. Satovic

Keywords

confusion probability, cultivar identification, discriminating capacity, RAPDs

Full text

https://doi.org/10.17660/ActaHortic.2004.663.115

Online Articles (181)

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56. THE EUROPEAN PROJECT HIDRAS: INNOVATIVE MULTIDISCIPLINARY APPROACHES TO BREEDING HIGH QUALITY DISEASE RESISTANT APPLES

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M. Ravelonandro | R. Scorza

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E. Chevreau | M. Faize | F. Dupuis | S. Sourice | L. Parisi

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81. OVER-EXPRESSION OF THE APPLE GENE MPNPR1 CAUSES INCREASED DISEASE RESISTANCE IN MALUS X DOMESTICA

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PH. Druart

84. EMBRYO RESCUE OF CHINESE JUJUBE (ZIZIPHUS JUJUBA MILL.)

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85. DEVELOPMENT OF A LEAF DISK REGENERATION SYSTEM FOR ‘BRAMLEY’S’ SEEDLING APPLE (MALUS X DOMESTICA BORKH.)

D. McAdam-O Connell | S. Mac An tSaoir | R. Copeland

86. IN VITRO MORPHOGENIC ABILITY OF MATURE OR EMBRYONIC APRICOT TISSUES

R. Paris | D. Pratesi | P. Negri

87. SUITABILITY OF SELECTED STRAWBERRY CULTIVARS FOR GENOME MODIFICATION BY AGROBACTERIUM TUMEFACIENS

A. Gruchała | M. Korbin | E. Żurawicz

88. AGROBACTERIUM-MEDIATED GENETIC TRANSFORMATION COMPETENCE OF DIFFERENT PRUNUS EXPLANTS, AS REVEALED BY GUS EXPRESSION

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89. TRANSGENIC APPLE CLONAL ROOTSTOCK RESISTANT TO BASTA HERBICIDE

S.V. Dolgov | K.G. Skryabin

90. THE USE OF MANNOSE AS A SELECTION AGENT IN TRANSFORMATION OF THE APPLE ROOTSTOCK M26 VIA AGROBACTERIUM TUMEFACIENS

L.H. Zhu | X.Y. Li | A. Ahlman | Z.T. Xue | M. Welander

91. APPLE TRANSFORMATION WITH THE GENE OF SUPERSWEET PROTEIN THAUMATIN II

S.V. Dolgov | K.A. Schestibratov | R.V. Mikhailov

92. THE LONG AND TORTUOUS PATH FROM THE LEAF PIECE TO THE GENETICALLY ENGINEERED APPLE TREE

V. Hanke

93. EVALUATION OF MALUS SIEVERSII SEEDLING POPULATIONS FOR DISEASE RESISTANCE AND HORTICULTURAL TRAITS

P.L. Forsline | H.S. Aldwinckle

94. EVALUATION OF MALUS SIEVERSII SEEDLINGS FROM KAZAKHSTAN FOR DISEASE RESISTANCE AND TIME OF LEAFING

D.D. Miller

95. MOLECULAR DIVERSITY IN POPULATIONS OF MALUS SIEVERSII (LEDED.) ROEM.

C. Wiedow | K.J. Dehmer | M. Geibel

96. ISOZYME POLYMORPHISM AND GENETIC STRUCTURE OF MALUS SYLVESTRIS (L.) MILL. NATIVE IN WESTERN AREAS OF GERMANY WITH RESPECT TO MALUS X DOMESTICA BORKH.

I. Wagner | H.P. Schmitt | W. Maurer | U. Tabel

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98. MOLECULAR GENETICS OF SELF-INCOMPATIBILITY IN PLUMS

B.G. Sutherland | K.R. Tobutt | T.P. Robbins

99. MAC-PR (MICROSATELLITE DNA ALLELE COUNTING USING PEAK RATIOS) REVEALS UNIQUE GENOMIC CONFIGURATION IN POLYPLOID DOGROSES

H. Nybom | G. Werlemark | G.D. Esselink | B. Vosman

100. SELECTION AND UTILIZATION OF MINOR FRUIT TREE SPECIES

V. Ognjanov | S. Cerović

101. SCREENING OF CHINESE JUJUBE GERMPLASM WITH HIGH RESISTANCE TO WITCHES’ BROOM DISEASE

M.J. Liu | J.Y. Zhou | J. Zhao

102. CONSERVATION OF OLD TRADITIONAL CHERRY LANDRACES IN THE CZECH REPUBLIC

F. Paprštein | J. Kloutvor | V. Holubec

103. INVENTORY ON FRUIT GENETIC RESOURCES IN SWITZERLAND

M. Kellerhals | S. Egger

104. GENETIC RESOURCES OF PRUNUS ARMENIACA L. IN THE CZECH REPUBLIC

B. Krška | Z. Vachůn | T. Nečas

105. DEVELOPMENT OF A SPECIFIC SOFTWARE FOR THE MANAGEMENT OF THE RECURRENT SYNONYMOUS PROBLEM OF CULTIVARS INSIDE PLANT GENETIC RESOURCES DATABASES: THE CASE OF THE EUROPEAN ECP/GR PYRUS DATABASE

R. Oger | M. Lateur

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M.L. Miaja | G. Gambino | R. Vallania | I. Gribaudo

107. WINTER HARDINESS IN THE U.S. DEPARTMENT OF AGRICULTURE MALUS CORE COLLECTION

J.J. Luby | D.S. Bedford | P.L. Forsline

108. TRADITIONAL OLD APPLE CULTIVARS AS NEW GENE SOURCES FOR APPLE BREEDING

M. Tóth | K. Kása | ZS. Szani | E. Balikó

109. EVALUATION OF APRICOT GERMPLASM IN SOUTHERN ITALY

G. Russo | L. D'Andrea

110. PHENOTYPIC DIVERSITY OF WILD CRANBERRY (VACCINIUM OXYCOCCOS) IN LITHUANIA

R. Daubaras | L. Chesoniene | J. Labokas

111. GENETIC DIVERSITY IN LINGONBERRY (ERICACEAE) REVEALED BY RAPD ANALYSIS

L. Garkava-Gustavsson

112. GENETIC DIVERSITY OF PRUNUS ROOTSTOCKS USING MICROSATELLITE MARKERS

M. Bouhadida | A.M. Casas | M.A. Moreno | Y. Gogorcena

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M. Foulongne | J. Minier | T. Pascal | J. Kervella

114. DEVELOPMENT OF MICROSATELLITE MARKERS IN FIG (FICUS CARICA L.)

E. Giraldo | M. López-Corrales | M.A. Viruel | J.I. Hormaza

115. MOLECULAR CHARACTERIZATION OF FRENCH LOCAL APPLE CULTIVARS USING SSRS

F. Laurens | C.E. Durel | M. Lascostes

116. EFFICIENCY OF RAPD MARKERS FOR VARIETAL IDENTIFICATION IN OLIVE (OLEA EUROPAEA L.) GERMPLASM COLLECTIONS

A. Belaj | L. Rallo | I. Trujillo | Z. Satovic

117. MOLECULAR CHARACTERIZATION OF APRICOT CULTIVARS AND NEW BREEDING LINES USING SSRS

D. Ruiz | R. Sánchez-Pérez | F. Dicenta | J. Egea | P. Marínez-Gómez

118. MOLECULAR AND HISTOCHEMICAL ANALYSIS OF VITIS VINIFERA L. CULTIVARS

119. GENOTYPING HUNGARIAN SWEET CHERRY CULTIVARS FOR SELF-INCOMPATIBILITY

Z. Bekefi | K.R. Tobutt | T. Sonneveld

120. EVALUATION OF DNA BASED TECHNIQUES FOR THE DETERMINATION OF SWEET CHERRY (PRUNUS AVIUM L.) SELF-INCOMPATIBILITY GENOTYPES

K.J. Williams | K.L. Willsmore | A.R. Granger

121. CHERRY (IN)COMPATIBILITY GENOTYPES – AN UPDATED CULTIVAR TABLE

K.R. Tobutt | T. Sonneveld | T. Bekefi | R. Bošković

122. GENETIC AND MOLECULAR CHARACTERISATION OF SELF-COMPATIBILITY IN ‘CRISTOBALINA’ SWEET CHERRY

A. Wünsch | J.I. Hormaza

123. SELF POLLINATION AND FERTILITY IN EUROPEAN PEAR (PYRUS COMMUNIS L.) CULTIVARS

P. Tassinari | S. Zuccherelli | S. Sansavini

124. GENETIC ALTERATIONS ASSOCIATED WITH THE ORIGINS OF FRUIT CULTURE

J. Janick

125. APPLE BREEDING AT CORNELL: GENETIC STUDIES OF FRUIT QUALITY, SCAB RESISTANCE AND PLANT ARCHITECTURE

S.K. Brown | K.E. Maloney | M. Hemmat | H.S. Aldwinckle

126. 75 YEARS OF TRADITION IN CLASSICAL PILLNITZ FRUIT BREEDING – AIMS, RESULTS

M. Fischer | C. Fischer

127. DEVELOPMENTS IN BREEDING AND TECHNIQUES WITHIN THE COMMUNITY PLANT VARIETY RIGHTS FRUIT SECTOR

S.F.A. Semon

128. APPLE BREEDING FOR DISEASE RESISTANCE IN LATVIA

L. Ikase | R. Dumbravs

129. SUITABILITY OF CLONE U 211 IN APPLE BREEDING FOR DISEASE RESISTANCE

E. Pitera

130. FIRE BLIGHT RESISTANT APPLE CULTIVARS PRODUCED BY CONVENTIONAL BREEDING

C. Fischer | K. Richter

131. IMPROVEMENT OF THE RESISTANCE TO SCAB, ROSY APPLE APHID AND FIRE BLIGHT IN A BREEDING PROGRAMME OF CIDER APPLE CULTIVARS

E. Dapena | M.D. Blázquez

132. RESULTS OF STUDY ON SOME BIOLOGICAL AND ECONOMICAL TRAITS OF NEW APRICOT SELECTIONS

A. Lyubenov | L. Ivanova

133. BREEDING STUDIES WITH ‘ÜSTÜN’ PEACH

E. Tanriver | A.B. Küden

134. SOME RESULTS OF A SWEET CHERRY BREEDING PROGRAMME IN BULGARIA

A. Zhivondov | P. Gercheva | V. Djouvinov

135. BREEDING BLACK CURRANTS WITH LATE FLOWERING AND RIPENING: SELECTION R/1/IX/87

M. Stanisavljevic

136. TEN YEARS OF OLIVE BREEDING IN CÓRDOBA (SPAIN)

L. León | R. de la Rosa | D. Barranco | L. Rallo

137. APPLE BREEDING FOR HIGH FRUIT QUALITY AND DURABLE DISEASE RESISTANCE

138. MARKER ASSISTED SELECTION FOR PL-1 POWDERY MILDEW RESISTANCE IN APPLE – OLD MARKERS FOR A NEW RESISTANCE GENE?

139. FRUIT SET AS AN EVALUATION CRITERION IN ALMOND BREEDING

R. Socias i Company | J.M. Alonso | J. Gómez Aparisi

140. POTENTIAL USE OF CHLOROPHYLL FLUORESCENCE IN FRUIT BREEDING

S. Khanizadeh | J.R. DeEll | D. Rekika

141. BUDBREAK NUMBER AS SELECTION CRITERION FOR BREEDING APPLES ADAPTED TO MILD WINTER CLIMATIC CONDITIONS: A REVIEW

I.F. Labuschagné

142. IMPROVEMENT OF SEED GERMINATION IN APPLE BREEDING

E. Pitera | S. Odziemkowski | B. Dabrowska

143. JUVENILE-ADULT CORRELATIONS IN PEAR, AND THEIR POSSIBLE UTILIZATION

S.H. Hjeltnes

144. SCREENING METHOD FOR POLYGENIC SCAB RESISTANCE WITHIN AN APPLE BREEDING PROGRAMME: RELATIONSHIP BETWEEN EARLY GREENHOUSE SCREENING TEST ON YOUNG SEEDLINGS AND THEIR SCAB SUSCEPTIBILITY IN NATURAL FIELD CONDITIONS

B. Lefrancq | M. Lateur | A. Rondia

145. SCREENING PRUNUS ROOTSTOCKS FOR TOLERANCE TO IRON CHLOROSIS

146. HOMOZYGOUS GENOTYPES FOR APPLE BREEDING: A FEASIBLE APPROACH?

L. Vanwynsberghe | J. Keulemans

147. A COMPREHENSIVE EVALUATION OF DH-MATERIAL IN APPLE

M.E. Höfer | C. Grafe | A. Boudichevskaia | A. Gomez | M.A. Bueno

148. MATERNAL HAPLOIDIZATION OF JAPANESE PEAR THROUGH INTER-GENERIC HYBRIDIZATION WITH APPLE

E. Inoue | F. Sakuma | M. Kasumi | H. Hara | T. Tsukihashi

149. ORIGIN OF ALMOND MULTIPLE EMBRYOS AND POTENTIAL UTILIZATION AS NEAR ISOGENIC LINES

R. Sánchez-Pérez | F. Dicenta | P. Martínez-Gómez | T.M. Gradziel

150. TARGET TRAITS FOR THE DEVELOPMENT OF MARKER ASSISTED SELECTION OF APPLE ROOTSTOCKS-PROSPECTS AND BENEFITS

G. Fazio | M. Mazzola

151. BREEDING FOR A NEW GENERATION OF PRUNUS ROOTSTOCKS BASED ON MARKER-ASSISTED SELECTION: A EUROPEAN INITIATIVE

152. POLYPLOID BREEDING AND THE POTENTIAL OF ITS USE FOR TRANSGENE CONTAINMENT WITH AMERICAN CRANBERRY (VACCINIUM MACROCARPON)

E.L. Zeldin | B.H. McCown

153. IDENTIFICATION AND EVALUATION OF DIPLOGYNY IN CLEMENTINE (CITRUS CLEMENTINA) FOR USE IN BREEDING

154. RE-EXAMINATION OF AN INTROGRESSION PROCEDURE OF A POLYGENIC RESISTANCE TO POWDERY MILDEW FROM WILD PRUNUS DAVIDIANA INTO PEACH IN THE LIGHT OF MOLECULAR DATA AND MONTE-CARLO SIMULATIONS

M. Foulongne | T. Pascal | F. Pfeiffer | J. Kervella

155. PROGRESS, PROSPECTS AND CHALLENGES IN A RECURRENT SELECTION PROGRAMME IN APPLE

N.C. Oraguzie | C. Whitworth | P.A. Alspach | C.G.T. Morgan | J. Fraser

156. INTERSPECIFIC HYBRIDISATION IN RIBES GENUS

V. Stanys | G. Stanienė | T. Shikshnianas | C. Bobinas

157. FIRST RESULTS IN THE ROMANIAN BREEDING PROGRAM OF ACTINIDIA ARGUTA

F. Stănică | C. Gavriluţ | M. Dumitraşcu | A.G. Peticilă | G. Zuccherelli

158. ‘FRIDA’ AND ‘FREDRIK’, THE FIRST SCAB-RESISTANT APPLE CULTIVARS DEVELOPED IN SWEDEN

H. Nybom

159. CONSUMER EVALUATION OF SCAB RESISTANT APPLE CULTIVARS

A.H. Jönsson

160. PRELIMINARY RESULTS ON THE PRODUCTION VALUE OF NEW SCAB-RESISTANT APPLE CULTIVARS BRED AT THE RESEARCH INSTITUTE OF POMOLOGY AND FLORICULTURE (RIPF), SKIERNIEWICE, POLAND

E. Żurawicz | M. Lewandowski | A. Broniarek-Niemiec | K. Rutkowski

161. SIX NEW APPLE CULTIVARS FROM DRESDEN-PILLNITZ

A. Peil | V. Hanke | C. Fischer

162. ‘REINETTE RUSSET^® AND ‘GALARINA’^®: TWO NEW WINTER HARDY DISEASE RESISTANT APPLE CULTIVARS RELEASED THROUGH JOINT COLLABORATION OF INRA (FRANCE) AND AAFC (CANADA)

163. BREEDING AND DEVELOPMENT OF THE NEW APPLE CULTIVAR ‘JAZZ ^TM‘

164. NEW APPLE AND PEAR SELECTIONS FROM HYBRID MATERIAL OF “IEDZENI” IN LATVIA

I. Drudze

165. NEW PEAR CULTIVARS FROM DRESDEN-PILLNITZ

M. Fischer | G. Mildenberger

166. ADAPTATION OF SOME JAPANESE PEARS IN HUNGARY

K. Honty | M. Göndör | Z. Boldog | Z. Horváth | Z. Szani

167. RECENT PEAR AND APRICOT INTRODUCTIONS FROM THE AAFC-HARROW TREE FRUIT BREEDING PROGRAMS

D.M. Hunter | R.E.C. Layne

168. RESULTS OF SOUR CHERRY BREEDING IN DRESDEN-PILLNITZ

M. Schuster | B. Wolfram

169. EVALUATION OF STRAWBERRY CULTIVARS AT THE POLLI HORTICULTURAL INSTITUTE (ESTONIA)

A. Kikas | A. Libek

170. FIELD PERFORMANCE OF SELECTED DOMESTIC AND FOREIGN STRAWBERRY CULTIVARS GROWN AT TWO SITES OF POLAND

E. Żurawicz | D. Kruczyńska | A. Masny | K. Pierzga

171. THREE NEW WINTER HARDY STRAWBERRY CULTIVARS ‘HARMONIE’, ‘SAINT-LAURENT D’ORLEANS’ AND ‘ST-JEAN D’ORLEANS’

172. ‘ORES’ AND ‘RUBEN’ – NEW BLACKCURRANT CULTIVARS BRED IN POLAN

S. Pluta | E. Żurawicz

173. GROWING GRAPES IN A COLD CLIMATE WITH WINTER TEMPERATURE BELOW -25°C

174. EVALUATION OF POLISH MUTANTS OF APPLE ROOTSTOCKS INDUCED WITH GAMMA RAYS

A.A. Przybyla | P. Bielicki | A. Czynczyk | D. Chlebowska

175. INFLUENCE OF NEW POLISH ROOTSTOCKS AND M.9 SUBCLONES ON INDUCED VIGOUR, YIELD AND FRUIT QUALITY OF APPLE CULTIVARS

P. Bielicki | A. Czynczyk | D. Chlebowska

176. GROWTH AND YIELD OF APPLES ON NEW CZECH DWARFING ROOTSTOCKS

J. Kosina

177. PRELIMINARY RESULTS FROM NEW PLUM ROOTSTOCKS TRIAL IN ESTONIA

H. Jänes | A. Pae

178. NEW POLISH CULTIVARS, ROOTSTOCKS AND INTERSTOCKS OF FRUIT TREES

Z.S. Grzyb | E. Rozpara

179. AGRONOMIC EVALUATION OF INRA PRUNUS ROOTSTOCKS FOR TWO JAPANESE PLUM CULTIVARS: ‘FRIAR’ AND ‘BLACK AMBER’

H. Duval

180. USE OF MYROBALAN SPECIES (PRUNUS CERASIFERA) AS ROOTSTOCKS FOR ALMOND AND PEACH

H. Duval | V. Signoret | R. Joly

181. RESULTS OF EIGHT YEARS OF RESEARCH ON THE GROWTH AND YIELD OF THREE SWEET CHERRY CULTIVARS GRAFTED ON ‘PHL A’ ROOTSTOCK

E. Rozpara | Z.S. Grzyb | B. Omiecińska | A. Czynczyk