GENOTYPING HUNGARIAN SWEET CHERRY CULTIVARS FOR SELF-INCOMPATIBILITY

The S-allele constitution of 21 sweet cherry (Prunus avium L.) cultivars important to Hungary was determined with PCR-based methods.
Two pairs of consensus primers that amplify the two variable intron regions of the gene coding for S-RNases and reveal length polymorphisms were used to amplify DNA extracted from buds.
For each cultivar, results were confirmed with allele-specific primer pairs.
The 21 cultivars had various pairs of 9 alleles including one new allele, S_x. Seventeen cultivars could be assigned to nine of the existing groups, two cultivars form a new group (S₄S₁₂) and two cultivars each had a new genotype, S₅S₉ and S₅S_x, and can be placed into group O. The cultivar grown in Hungary under the name ‘Hedelfingeni Orias’ was found to have the genotype S₃S₄, and not S₃S₅ as previously reported for the cultivar ‘Hedelfingen’. Therefore we propose distinguishing this clone as ‘Hedelfingen O’.

XI Eucarpia Symposium on Fruit Breeding and Genetics

Z. Bekefi, K.R. Tobutt, T. Sonneveld

Prunus avium, incompatibility, S-alleles

https://doi.org/10.17660/ActaHortic.2004.663.118