Articles
GENETIC DIVERSITY OF NEPHELIUM RAMBOUTAN-AKE LEENH. ASSESSED USING RAPD AND ISSR MARKERS
Article number
665_19
Pages
171 – 182
Language
English
Abstract
Genetic variation among Nephelium ramboutan-ake accessions maintained in field genebanks at MARDI Serdang and at MARDI Kemaman was assessed using random amplified polymorphic DNA (RAPD) and inter-simple sequence repeat (ISSR) markers.
Values of genetic diversity based on Shannon index and genetic relatedness based on Jaccard index of genetic similarity differed depending on the groups of accessions surveyed and markers used.
For 24 accessions from MARDI Kemaman ISSRs detected less polymorphism (87.10%) compared to RAPDs (97.33%). Besides, RAPDs also revealed higher genetic diversity (H0 5.45) than ISSRs (H0 1.99) with mean Jaccard index of genetic similarity 0.408 (RAPDs) and 0.561 (ISSRs) respectively.
This study illustrates how choice of DNA markers can result in different estimates of genetic diversity and values of relatedness in N. ramboutan-ake.
Values of genetic diversity based on Shannon index and genetic relatedness based on Jaccard index of genetic similarity differed depending on the groups of accessions surveyed and markers used.
For 24 accessions from MARDI Kemaman ISSRs detected less polymorphism (87.10%) compared to RAPDs (97.33%). Besides, RAPDs also revealed higher genetic diversity (H0 5.45) than ISSRs (H0 1.99) with mean Jaccard index of genetic similarity 0.408 (RAPDs) and 0.561 (ISSRs) respectively.
This study illustrates how choice of DNA markers can result in different estimates of genetic diversity and values of relatedness in N. ramboutan-ake.
Authors
M.M. Clyde, P.C. Chew, I. Salma, M.N. Normah, V.R. Rao
Keywords
genetic relatedness, DNA markers, Shannon index, Jaccard index, genetic similarity, polymorphism
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