Articles
ANTI-ANGIOGENIC, ANTI-INFLAMMATORY AND ANTI-OXIDANT POTENTIAL OF AN AFRICAN RECIPE: ALCHORNEA CORDIFOLIA SEEDS
Article number
678_11
Pages
91 – 96
Language
English
Abstract
The chorio-allantoic membrane (CAM) and hens egg test (HET-CAM) assays are based on neo-vascularisation (angiogenesis) in fertilised henss egg embryo.
Therefore inhibition of angiogenesis is a prime target for solutions to afflictions such as growth of solid tumours, arthrithis, chronic inflammations.
Natural products still represent an untapped source of interesting leads for drug development against these diseases.
Serial dilutions of the methanolic extract of Alchornea cordifolia seeds were assayed on CAM, HET-CAM and 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) assays.
The results revealed an inhibition of angiogenesis at 0.9 score and 86% inhibition of inflammation at 250 µg/pellet on the CAM and HET-CAM respectively.
It was also very effective in the DPPH assay for non specific hydrogen atom or electron donating activity (IC50: 0.68 µg/ml). Subsequent fractionations, revealed the ethyl acetate fraction (Ea) as the most active against others in a dose response trend.
Interestingly, no side effects such as embryotoxic effects and others were recorded.
The findings support the folkloric uses of the plant against wounds, piles and cancer and therefore have implications on the quality of herbal drugs dispensed by the traditional medical practitioners (TMP) in Africa.
Therefore inhibition of angiogenesis is a prime target for solutions to afflictions such as growth of solid tumours, arthrithis, chronic inflammations.
Natural products still represent an untapped source of interesting leads for drug development against these diseases.
Serial dilutions of the methanolic extract of Alchornea cordifolia seeds were assayed on CAM, HET-CAM and 2,2-diphenyl-1-picrylhydrazyl hydrate (DPPH) assays.
The results revealed an inhibition of angiogenesis at 0.9 score and 86% inhibition of inflammation at 250 µg/pellet on the CAM and HET-CAM respectively.
It was also very effective in the DPPH assay for non specific hydrogen atom or electron donating activity (IC50: 0.68 µg/ml). Subsequent fractionations, revealed the ethyl acetate fraction (Ea) as the most active against others in a dose response trend.
Interestingly, no side effects such as embryotoxic effects and others were recorded.
The findings support the folkloric uses of the plant against wounds, piles and cancer and therefore have implications on the quality of herbal drugs dispensed by the traditional medical practitioners (TMP) in Africa.
Authors
R. Nia, D.H. Paper, G. Franz, E.E. Essien
Keywords
CAM, HET-CAM, DPPH assays, inflammation, neo-vascularisation
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