Articles
ANTI-OXIDANT AND ANTI-INFLAMMATORY ACTIVITY OF MANNIOPHYTON FULVUM
Article number
678_12
Pages
97 – 101
Language
English
Abstract
Manniophyton fulvum Muell.
Arg (Euphorbiaceae), is used for wound healing, inflammatory conditions and against dysentery, and diarrhea in central Africa.
In addition, the plant is an important delicacy for the pygmy chympanzee (Pan paniscus). Up to now, no scientific proof exists to justify such practices.
To unravel the rationale behind the folkloric uses, the leaves of the plant were extracted in methanol and chemically analysed for its secondary metabolites.
The crude methanolic extract was assayed for its anti-oxidant activity using 2,2-diphenyl-1-picrylhydrazyl hydrate:DPPH assay and for its anti-inflammatory potential using hens eggs test-chorioallantoic membrane assay: HET-CAM. The crude methanolic extract showed a significant anti-oxidant activity (IC50: 1.06 µg/ml) as well as a strong dose dependent anti-inflammatory activity (92% inhibition at 250 µg/pellet). Via bio-assay fractionation, the main activity can be enriched in the medium polar fraction.
Interestingly, no embryotoxic effects and or membrane irritation was observed in the purified fraction.
These results clearly demonstrate the rationale behind the uses of this plant against the ascribed ailments, both by the traditional medical practitioners (TMP) in Africa and by Pan paniscus as a food component.
Arg (Euphorbiaceae), is used for wound healing, inflammatory conditions and against dysentery, and diarrhea in central Africa.
In addition, the plant is an important delicacy for the pygmy chympanzee (Pan paniscus). Up to now, no scientific proof exists to justify such practices.
To unravel the rationale behind the folkloric uses, the leaves of the plant were extracted in methanol and chemically analysed for its secondary metabolites.
The crude methanolic extract was assayed for its anti-oxidant activity using 2,2-diphenyl-1-picrylhydrazyl hydrate:DPPH assay and for its anti-inflammatory potential using hens eggs test-chorioallantoic membrane assay: HET-CAM. The crude methanolic extract showed a significant anti-oxidant activity (IC50: 1.06 µg/ml) as well as a strong dose dependent anti-inflammatory activity (92% inhibition at 250 µg/pellet). Via bio-assay fractionation, the main activity can be enriched in the medium polar fraction.
Interestingly, no embryotoxic effects and or membrane irritation was observed in the purified fraction.
These results clearly demonstrate the rationale behind the uses of this plant against the ascribed ailments, both by the traditional medical practitioners (TMP) in Africa and by Pan paniscus as a food component.
Authors
R. Nia, D.H. Paper, G. Franz, E.E. Essien, M. Muganza, G. Hohmann
Keywords
HET-CAM, DPPH assay, fractionation, Pan paniscus, dose response
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