Articles
EFFECTS OF THE ESSENTIAL OILS FROM JUNIPERUS RIGIDA SIEB ET. ZUCC. ON GROWTH PERFORMANCE OF RAT ADRENAL NERVE CELLS
Article number
678_17
Pages
127 – 134
Language
English
Abstract
It was proved that the composition of the oils by conventional steam distillation extraction (SDE) process was much different from that obtained by supercritical fluid extraction (SFE) process using carbon dioxide.
The oils from two processes extracted contain similar amounts of three major components such as α-pinene, myrcene and terpinolene, however, the oils by SFE had higher amounts of fatty acids and only citronellol.
The extraction yield by SFE method was higher than by SDE, maintaining 0.054% (v/v) vs 0.032% (v/v), respectively.
In general, the oils from SFE process can show better growth performance of nerve cells such as cell growth, initiation and elongation of the neurites, followed by that from SDE, compared to the results without supplementation (control). It was interesting that the oils could play an important role in enhancing the initiation of the neurites as well as improving the growth of the nerve cells.
It was also found that the oils by SFE took only 20 min to react with the cells while the control took 160 min by kinetic analysis of the oils responding to the cells.
The oils extracted by SFE definitely delayed apoptotic death of nerve cells, which could result in maintaining better cell growth and higher elongation of the neurites of the cell.
The oils from two processes extracted contain similar amounts of three major components such as α-pinene, myrcene and terpinolene, however, the oils by SFE had higher amounts of fatty acids and only citronellol.
The extraction yield by SFE method was higher than by SDE, maintaining 0.054% (v/v) vs 0.032% (v/v), respectively.
In general, the oils from SFE process can show better growth performance of nerve cells such as cell growth, initiation and elongation of the neurites, followed by that from SDE, compared to the results without supplementation (control). It was interesting that the oils could play an important role in enhancing the initiation of the neurites as well as improving the growth of the nerve cells.
It was also found that the oils by SFE took only 20 min to react with the cells while the control took 160 min by kinetic analysis of the oils responding to the cells.
The oils extracted by SFE definitely delayed apoptotic death of nerve cells, which could result in maintaining better cell growth and higher elongation of the neurites of the cell.
Authors
M.K. Kee, H.Y. Lee, N.S. Sung, J.C. Ahn, B. Hwang
Keywords
percrome nerve cells, neurites, apoptosis, apoptotic death, volatile essential oils
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