Articles
POSTULATED MECHANISMS OF ACTION OF CARDIOPROTECTIVE DRUG TERMINALIA ARJUNA: EFFECT OF TRITERPENOIDAL CONSTITUENTS ON THE PROCESS OF RESPIRATORY OXYBURST
Article number
678_15
Pages
115 – 118
Language
English
Abstract
Terminalia arjuna is an important medicinal plant widely used in the preparation of Ayurvedic formulations used in cardioprotection.
Only one sapogenin acid i.e. arjunolic acid has been shown to provide cardiac protection in induced necrotic rats but no other report for the biological activity of any of the isolated components is available.
The present work is to rationalize all saponins for their biological effects using oxidative mechanisms as reactive oxygen species (ROS) implicated in many pathogenic processes including the cardiovascular system.
Two sapogenin namely arjungenin and arjunic acid and their respective glucosides arjunetin and arjunglucoside II, isolated from the alcoholic extract of bark of Terminalia arjuna were tested for their action on the free radical scavenging action using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay, effect on the superoxide release from PMN cells using NBT (Nitroblue tetrazolium) reduction assay, and hypochlorous acid release from human neutrophils using luminol enhanced chemiluminescence assay.
Arjungenin was found to be most active as direct free radical scavenger and inhibitor for the hypochlorous acid production followed by its glucoside that was almost 50% active.
Only one sapogenin acid i.e. arjunolic acid has been shown to provide cardiac protection in induced necrotic rats but no other report for the biological activity of any of the isolated components is available.
The present work is to rationalize all saponins for their biological effects using oxidative mechanisms as reactive oxygen species (ROS) implicated in many pathogenic processes including the cardiovascular system.
Two sapogenin namely arjungenin and arjunic acid and their respective glucosides arjunetin and arjunglucoside II, isolated from the alcoholic extract of bark of Terminalia arjuna were tested for their action on the free radical scavenging action using DPPH (1,1-diphenyl-2-picrylhydrazyl) assay, effect on the superoxide release from PMN cells using NBT (Nitroblue tetrazolium) reduction assay, and hypochlorous acid release from human neutrophils using luminol enhanced chemiluminescence assay.
Arjungenin was found to be most active as direct free radical scavenger and inhibitor for the hypochlorous acid production followed by its glucoside that was almost 50% active.
Authors
R.S. Pawar, C. Gopalakrishnan, K.K. Bhutani
Keywords
Terminalia arjuna, respiratory oxyburst, cardioprotection
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