Articles
EFFECT OF BRASSICA CARINATA SEED MEAL TREATMENT ON THE TRICHODERMA HARZIANUM T39-SCLEROTINIA SPECIES INTERACTION
Article number
698_38
Pages
287 – 292
Language
English
Abstract
The fungal biocontrol agents and the biofumigation with plants containing glucosinolates are known to reduce soil plant pathogens and are alternative methods to chemical plant disease control measure. Brassica carinata seed meal contained sinigrin as major glucosinolate.
In vitro tests showed that Sclerotinia sclerotiorum and Sclerotinia minor were more sensitive than Trichoderma harzianum-T39 to isothiocyanate released from sinigrin.
In fact only T. harzianum-T39 is able to grow in presence of 5 µmoles glucosinolate per plate, the highest concentration tested.
In the presence of meal, colonies showed a lag-phase period which length was affected by glucosinolate concentration, a reduction of the mycelial growth and delay of the sclerotia germination.
When T. harzianum-T39 and B. carinata seed meal were used in combination, the antagonistic activity of the biocontrol agent against the plant pathogens was not reduced in terms of colonization ability, the efficacy of diffusible and volatile metabolites released from the antagonist.
Even if the presence of volatile metabolites determined a negative charge on the isothiocyanate efficacy, on the whole the treatment by B. carinata seed meal can be considered compatible with the application of the antagonistic fungus T. harzianum-T39.
In vitro tests showed that Sclerotinia sclerotiorum and Sclerotinia minor were more sensitive than Trichoderma harzianum-T39 to isothiocyanate released from sinigrin.
In fact only T. harzianum-T39 is able to grow in presence of 5 µmoles glucosinolate per plate, the highest concentration tested.
In the presence of meal, colonies showed a lag-phase period which length was affected by glucosinolate concentration, a reduction of the mycelial growth and delay of the sclerotia germination.
When T. harzianum-T39 and B. carinata seed meal were used in combination, the antagonistic activity of the biocontrol agent against the plant pathogens was not reduced in terms of colonization ability, the efficacy of diffusible and volatile metabolites released from the antagonist.
Even if the presence of volatile metabolites determined a negative charge on the isothiocyanate efficacy, on the whole the treatment by B. carinata seed meal can be considered compatible with the application of the antagonistic fungus T. harzianum-T39.
Publication
Authors
S. Sanchi, S. Odorizzi, L. Lazzeri, P. Marciano
Keywords
biofumigation, glucosinolate, isothiocyanate, biocontrol agent, antagonistic activity, Sclerotinia sclerotiorum, Sclerotinia minor
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