Home > V International Symposium on In Vitro Culture and Horticultural Breeding > PRODUCTION OF DOUBLED HAPLOID BREEDING LINES IN CASE OF PAPRIKA, SPICE PAPRIKA, EGGPLANT, CUCUMBER, ZUCCHINI AND ONION

Articles

PRODUCTION OF DOUBLED HAPLOID BREEDING LINES IN CASE OF PAPRIKA, SPICE PAPRIKA, EGGPLANT, CUCUMBER, ZUCCHINI AND ONION

Article number

725_117

Pages

845 – 854

Language

English

Abstract

Doubled haploids are nowadays a routine part of the breeding programmes and the fastest way to get complete homozygote breeding material.
Breeding programmes require large numbers of genetically stable plants.
If a sufficient quantity of dihaploid plants are to be produced for use in breeding, the efficiency of in vitro techniques must be continuously improved.
The main results we achived in in vitro androgenesis and gynogenesis as follows.
In anthera cultures of paprika and eggplants the inclusion of maltose in the induction medium improved the induction of microspore embryogenesis and rised the number of regenerant plants.
An efficient, reproducible and economic method was elaborated for the in vitro genome duplication of paprika and eggplants haploids.
In in vitro flower-bud and ovary cultures of onion on the basis of our results it could be concluded that application of thidiazuron not only improved embryo induction but increased in the ratio of spontaneous doubled haploids.
In case of cucumber and zucchini a reproducible, novel method was elaborated for the induction of in vitro gynogenesis.

Publication

V International Symposium on In Vitro Culture and Horticultural Breeding

Authors

A. Gémes Juhasz, G. Venczel, ZS. Sagi, L. Gajdos, Z. Kristof, P. Vagi, L. Zatyko

Keywords

haploids, doubled haploids, androgenesis, gynogenesis, genome doubling

Full text

https://doi.org/10.17660/ActaHortic.2006.725.117

Online Articles (120)

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4. PLANT BIOTECHNOLOGY IN TURKEY

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5. SOME ASPECTS OF EMBRYO DEVELOPMENT IN VITRO

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6. THE APPLICATION OF BIOTECHNOLOGY IN AN INTEGRATED PROJECT OF CONSERVATION AND UTILIZATION OF PAPAYA AND ITS WILD RELATIVES

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7. REGENERATION-PROMOTING GENES IMPROVE TRANSFORMATION EFFICIENCY IN APRICOT

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14. IN VITRO PROPAGATION OF CAPPARIS SPINOSA

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15. IN VITRO MORPHOGENESIS OF SHOOTS FROM LEAF EXPLANTS OF CUCUMBER (CUCUMIS SATIVUS L.)

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16. INCREASE IN THE QUALITY OF RASPBERRY CULTURES UNDER THE INFLUENCE OF FEEDDHA

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17. IN VITRO REGENERATION OF ALLIUM AFLATUNENSE BY SOMATIC EMBRYOGENESIS

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18. IN VITRO PLANT REGENERATION IN ZYGOTIC EMBRYO CULTURE OF IRIS REICHENBACHII

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19. AGAR PHENOLIC IMPURITIES AFFECT THE BIOLOGICAL PERFORMANCE OF IN VITRO CULTURES

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20. IMPACT OF SODIUM-SELENATE ON THE GROWTH OF RADISH SEEDLINGS IN VITRO

21. PROTOPLAST CULTURE IN PETUNIA HYBRIDA AND EFFECTS OF CELL CYCLE INHIBITORS ON CELL DIVISION FREQUENCY

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22. HOW CAN DIFFERENT CYTOKININS INFLUENCE THE PROCESS OF SHOOT REGENERATION FROM APPLE LEAVES IN ´ROYAL GALA´ AND ´M.26´

23. EFFECTS OF CARBON AND NITROGEN SOURCES ON PROTOCORM-LIKE BODIES PROLIFERATION OF DENDROBIUM HUOSHANENSE BY LIQUID CULTURE

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24. ANALYSIS OF SUITABILITY OF DNA EXTRACTION PROTOCOLS FOR SOMACLONAL VARIATION ANALYSIS IN IN VITRO-CULTURED ORCHIDS

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25. BREEDING OF ROSA RUGOSA THUNB. USING DIFFERENT TISSUE CULTURE METHODS

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28. A COMPARATIVE HISTOLOGICAL STUDY BETWEEN ´EMBRYOGENIC STRUCTURES´ AND ZYGOTIC EMBRYOS OF CLEMATIS HYBRIDS CULTIVATED IN VITRO

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31. EMERGING THEMES IN IN VITRO CULTURE AND THEIR APPLICATIONS TO HORTICULTURE

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32. ANEUPLOIDY, A SOURCE OF GENETIC DIVERSITY FOR FRUIT SPECIES

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33. NEW AROMATIC CYTOKININS CAN MAKE THE DIFFERENCE

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36. MICROPROPAGATION INDUSTRY IN INDIA: BIOLOGY AND BUSINESS

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37. HEALTHY IN VITRO PROPAGATION BY MERISTEM TIP CULTURE OF HELLEBORUS NIGER¿S SELECTED CLONE FOR CUT FLOWER

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38. BIOTECHNOLOGIES FOR THE PRESERVATION OF SELECTED RED CHICORY (CICHORIUM INTYBUS L.) LINES

39. ELIMINATION AND DETECTION OF PATHOGENS FROM TISSUE CULTURES OF PRUNUS SP.

40. GROWTH AND PHYSIOLOGICAL CHARACTERISTICS OF PLANTLETS OF SPATHIPHYLLUM ´SENSATION´ IN ROOTING PHASE AS AFFECTED BY SUCROSE-FREE MEDIUM AND DIFFUSIVE VENTILATION

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41. IN VITRO GERMINATION OF THREE OLIVE CULTIVARS

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42. SOMACLONAL VARIATION INDUCED DE NOVO LEAF CHIMERIC MUTANTS DURING IN VITRO PROPAGATION OF AFRICAN VIOLET (SAINTPAULIA IONANTHA WENDL.)

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43. MICROPROPAGATION OF STEMONA CURTISII HOOK F., A THAI MEDICINAL PLANT

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44. THE INFLUENCE OF LIGHT COLOUR ON MICROPROPAGATION OF CHRYSANTHEMUM

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45. CRYOPRESERVATION OF ROSA SHOOT TIPS: IMPORTANCE OF PRECULTURE CONDITIONS

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46. CONSERVATIVE MICROPROPAGATION OF SOME ENDEMIC OR RARE SPECIES FROM THE DIANTHUS GENUS

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47. MICROPROPAGATION OF HORSERADISH (ARMORACIA RUSTICANA)

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48. APPLICATION OF SILICON RUBBER GRAFTING CLIPS FOR IN VITRO PHYTOPATHOLOGICAL STUDIES

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49. PROTOCORM-LIKE BODY FORMATION AND PLANT REGENERATION OF DENDROBIUM HUOSHANENSE, AN ENDANGERED MEDICINAL PLANT

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50. THE EFFECT OF INHERITING FACTOR ON MINERAL NUTRITION OF LOW VIGOROUS SWEET CHERRY ROOTSTOCKS

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51. CRYOPRESERVATION OF EMBRYOGENIC SUSPENSION CULTURES OF CYCLAMEN PERSICUM MILL.

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52. PLANT REGENERATION FROM DIFFERENT EXPLANT TYPES OF STREPTOCARPUS SPEC. IN VITRO

R. Afkhami-Sarvestani | M. Serek | T. Winkelmann

53. MORPHOLOGICAL VARIATION OF CORMS IN PLANTS REGENERATED FROM CALLUSES OF TARO (COLOCASIA ESCULENTA SCHOTT)

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54. IN VITRO PROPAGATION AND MULTIPLE SHOOT FORMATION FROM COTYLEDONARY NODES OF PISTACHIO

Y. Ozden-Tokatli | E.A. Ozudogru | A. Akcin

55. EFFECT OF MANNITOL AND ABA ON INDUCTION OF SOMATIC EMBRYOGENESIS IN TEA (CAMELLIA SINENSIS L.)

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56. EFFECT OF FRUIT CRYOPRESERVATION ON IN VITRO GERMINATION OF ZYGOTIC EMBRYOS OF SEVERAL SPECIES OF ILEX

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57. STIMULATION OF IN VITRO BULBLET GROWTH IN GALANTHUS SPECIES WITH SUCROSE AND ACTIVATED CHARCOAL

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58. IN VITRO PROPAGATION OF DIANTHUS BALBISII SER. SUBSP. LIBURNICUS (BARTL.) PIGN. BY SHOOT TIP CULTURE

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59. OPTIMIZATION OF ROOTING OF IN VITRO PROPAGATED MALUS X DOMESTICA ¿PINOVA¿

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60. THE INFLUENCE OF GROWTH RETARDANTS AND BA ON THE GROWTH AND DEVELOPMENT OF TIBOUCHINA URVILLEANA COGN. IN VITRO

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61. RESULTS WITH THE MICROPROPAGATION OF GALANTHUS ELWESII AND GALANTHUS NIVALIS ´FLORE PLENO´

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62. IN VITRO METHODS FOR TESTING POTATO CLONES AGAINST SOFT ROT ERWINIAE

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63. INTERSPECIFIC HYBRIDIZATION IN GENUS FRAGARIA AND EVALUATION OF THE HYBRIDS FOR COLD HARDINESS AND RED STELE RESISTANCE USING IN VITRO METHODS

R. Rugienius | D. Gelvonauskiene | I. Zalunskaite | J. Pakalniskyte | V. Stanys

64. MICROPROPAGATION OF ´ECHO´ CULTIVARS OF EUSTOMA GRANDIFLORUM

M. Ordogh | E. Jambor-Benczur | A. Tilly-Mandy

65. EFFECT OF PROPAGATION METHOD ON PLANT DEVELOPMENT AND CONTENT OF BIOLOGICALLY ACTIVE COMPOUNDS IN THE HERB OF ST. JOHN´S WORT (HYPERICUM PERFORATUM)

A. Pawelczak | E. Osinska | A. Geszprych | Z. Weglarz

66. PROLIFERATION AND COLD HARDENING OF IN VITRO GROWN APPLE SHOOT TIPS

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67. EFFECTS OF TEMPERATURE ON THE GROWTH AND DORMANCY OF TISSUE-CULTURED HERBACEOUS PEONY SHOOTS

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68. IN VITRO MORPHOGENESIS IN ZYGOTIC EMBRYO CULTURES OF CAROB TREE (CERATONIA SILIQUA L.)

L. Custodio | A. Romano

69. IN VITRO SLOW GROWTH STORAGE OF FRUIT ROOTSTOCKS INSIDE GAS-TIGHT OR GAS-PERMEABLE CONTAINERS

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73. LONG TERM SURVIVAL OF CRYPTIC VIRUSES IN ASEPTICALLY GROWN IN VITRO PROPAGATED PLANTS

A. Szego | P. Ilyes | E.K. Toth | L. Potvondi | N. Lukacs

74. MICROPROPAGATION OF PEACH ROOTSTOCKS AND CULTIVARS

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75. EFFECT OF CULTURE VESSELS ON THE BIOMASS PRODUCTION AND TOTAL ANTIOXIDANT CAPACITY IN PEA SEEDLINGS GERMINATED UNDER PAM-SYSTEM

76. OPTIMIZATION OF TISSUE CULTURE MEDIA FOR PROPAGATION OF MARIANNA ROOTSTOCKS

E.L. Mansvelt | L. Watts | M.H. Spreeth | A. Meszaros

77. BIOTECHNOLOGY OF ANNUAL FLOWER PLANTS: MICROPROPAGATION OF RUDBECKIA SP.

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78. COST EXPERIENCES WITH BIOREACTORS

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79. DESIGN OF A REVERT ROTARY PLANT MICROPROPAGATION BIOREACTOR ¿ ´3R´ SYSTEM: APPLICATION FOR RESEARCH, POSSIBILITIES OF SCALING-UP AND LIMITATIONS

M.G. Fari | T. Kertesz | M. Laszlo | ZS. Varga

80. MASS PROPAGATION OF COFFEE TRANSPLANTS UNDER SCALED-UP PHOTOAUTOTROPHIC MICROPROPAGATION SYSTEM

F. Afreen | S.M.A. Zobayed | T. Kozai

81. MASS PROPAGATION OF CHERRY (CERASUS × YEDOENSIS MATSUM.) THROUGH SHOOT PRIMORDIA

82. APPLICATION OF TISSUE CULTURE AND MOLECULAR FARMING IN THE PRODUCTION OF PHARMACEUTICALS

S. Sorvari | M.G. Fari | M. Laszlo | O. Toldi

83. APPLICATION OF THE TEMPORARY IMMERSION SYSTEM FOR THE IN VITRO PRODUCTION OF BIOACTIVE COMPOUNDS IN HARPAGOPHYTUM (DEVIL’S CLAW)

84. COMPARISON OF GROWTH AND SECONDARY METABOLITE ACCUMULATION IN CULTURES OF COMPACT CALLUS AGGREGATES AND SHOOTS OF HYPERICUM PERFORATUM L. IN SHAKE FLASKS AND IN A BUBBLE COLUMN BIOREACTOR

85. ENHANCING THE BIOTRANSFORMATION RATE OF RHODIOLA ROSEA CALLUS CULTURES

Z. Gyorgy | P. Neubauer | A. Tolonen | A. Hohtola

86. AN EFFICIENT MICROPROPAGATION SYSTEM: 3R BIOREACTOR

A. Mészaros | E. Domokos-Szabolcsy | K. Halasz | K. Kalai | M.G. Fari

87. APPLICATION OF SCAR MARKERS LINKED TO A PUTATIVE PRSV-P RESISTANCE LOCUS FOR ASSISTED BREEDING OF RESISTANT C. PAPAYA CULTIVARS

S. Dillon | C. Ramage | M. O'Brien | R.A. Drew

88. IDENTIFICATION OF GENES INVOLVED IN RIPENING OF OCTOPLOID STRAWBERRY (FRAGARIA X ANANASSA DUCH.) USING CDNA-AFLP

A. Balogh | E. Kiss | T. Koncz | L. Heszky

89. GAMMA RAYS INDUCED IN VITRO MUTAGENESIS AND MOLECULAR MARKER-ASSISTED SELECTION OF MUTANTS IN GRAPEVINE

R.N. Khawale | S.K. Singh | Y. Vimala

90. IN VITRO CULTURE AND GENETIC ANALYSIS OF MALE AND FEMALE DATE PALM (PHOENIX DACTYLIFERA L.)

N.S. Al-Khalifah | F.A. Khan | E. Askari | S. Hadi

91. NIR – AN ALTERNATIVE TOOL IN HORTICULTURAL BIOTECHNOLOGY

92. USING SSR MARKERS TO DISTINGUISH APPLE CULTIVARS

Z. Galli | G. Halasz | E. Kiss | J. Dobranszki | L. Heszky

93. AGGRESSIVENESS OF PHYTOPHTHORA INFESTANS ISOLATES CORRELATES WITH THEIR PROTEOLYTIC ACTIVITY

J.A. Hamill | C. Selby | L.R. Cooke

94. IMPROVEMENT OF BULB FORMATION IN MICROPROPAGATED TULIPS BY TREATMENT WITH NAA AND PACLOBUTRAZOL OR ANCYMIDOL

M. Podwyszynska

95. POTENTIAL RAPD APRICOT MARKER LINKED TO PPV RESISTANCE

M. Vachun | M. Kafonkova | M. Pidra | J. Raddova | B. Krska

96. CHARACTERIZATION OF DIFFERENT APRICOT CULTIVARS GROWN IN HUNGARY WITH SSR MARKERS

A. Pedryc | SZ. Ruthner | GY.D. Bisztray | M. Laimer

97. GENOTYPE IDENTIFICATION OF ONION (ALLIUM CEPA L.) CULTIVARS

98. DISTINGUISHING OF THE GRAPEVINE CULTIVARS ´PICOLIT´ AND ´KÉKNYELŰ´ WITH THE HELP OF IZOENZYME ANALYSES

G. Györffyné Jahnke

99. NIR SPECTROSCOPY FOR DISTINCTION OF HORTICULTURAL PLANT SEEDS

GY.D. Bisztray | T. Deak | ZS. Seregély | K. Kaffka

100. MORPHOLOGICAL AND GENETIC INVESTIGATION OF MEDIAEVAL GRAPE SEEDS

101. PATENTS AND TRANSGENIC PLANTS

J.M. Dunwell

102. A REAL THREAT OF MANIPULATION

E. Balazs

103. AGROBACTERIUM-MEDIATED TRANSFORMATION OF PELARGONIUM (PELARGONIUM ZONALE HYBRIDS AND PELARGONIUM PELTATUM HYBRIDS)

T. Winkelmann | K. Kayiani | M. Serek

104. TRANSFORMATION OF WOODY SPECIES: STATE OF THE ART

105. PHYTOREMEDIATION WITH TRANSGENIC PLANTS

106. A SELECTION STRATEGY TO OBTAIN TRANSFORMED APRICOT PLANTS

C. Petri | S. Lopez-Noguera | L. Burgos | N. Alburquerque

107. ASSESSMENT OF REGENERATION AND TRANSFORMATION IN SWEET CHERRY CULTIVARS

M.C. Piagnani | A. Scotti

108. TRICHODERMA CHITINASE GENE EXPRESSION CONFERS MOULD RESISTANCE

K. Kalai | G. Giczey | A. Mészaros | F. Dénes | E. Balazs

109. EXPERIMENTS ON TRANSFORMATION AND IN VITRO REGENERATION OF SQUASH VARIETIES

110. TOWARDS AGROBACTERIUM-MEDIATED TRANSFORMATION OF HIPPEASTRUM X CHMIELII CHM. ¿ IDENTIFICATION OF APPROPRIATE SELECTION MEDIA

A. Ilczuk | A. Lukaszewska | T. Winkelmann | M. Serek

111. ELECTROFUSION OF PROTOPLASTS IN SELECTED VEGETABLES OF BRASSICA, CUCUMIS AND SOLANUM GENERA

112. EFFECTS OF MODIFIED CARBOHYDRATE CONTENT ON THE DEVELOPMENT AND GROWTH OF CARNATION (DIANTHUS CARYOPHYLLUS L.)

A. Szoke | E. Kiss | I. Kerepesi | O. Toldi | L. Heszky

113. TRANSFORMATION OF APPLE (MALUS DOMESTICA BORKH.) USING THE PHOSPHOMANNOSE ISOMERASE GENE AS A SELECTABLE MARKER

J. Degenhardt | I. Szankowski

114. HAPLOID PRODUCTION AND CHROMOSOME DOUBLING

K.J. Kasha | Y.S. Shim | E. Simion | J. Letarte

115. DEVELOPMENT OF DOUBLE HAPLOIDY IN UMBELLIFERAE

A.M.R. Ferrie | T.D. Bethune | D. Watere

116. CUCUMBER (CUCUMIS SATIVUS L.) DIHAPLOID LINE PRODUCTION USING IN VITRO RESCUE OF IN VIVO INDUCED PARTHENOGENIC EMBRYOS

R. Dolcet-Sanjuan | E. Claveria | J. Garcia-Mas

117. PRODUCTION OF DOUBLED HAPLOID BREEDING LINES IN CASE OF PAPRIKA, SPICE PAPRIKA, EGGPLANT, CUCUMBER, ZUCCHINI AND ONION

118. ANDROGENESIS INDUCTION FROM TOMATO ANTHER CULTURES: CALLUS CHARACTERIZATION

J.M. Segui-Simarro | F. Nuez

119. CRYOPRESERVATION OF POLLEN OF CAROB TREE

L. Custodio | N. Fernandes | M.F. Carneiro | A. Romano

120. FLAX PLANTS REGENERATED FROM UNPOLLINATED OVULES CULTURED IN OVARY SEGMENTS

Z. Bartosova | S. Masar | A. Pretova