Home > V International Symposium on In Vitro Culture and Horticultural Breeding > A COMMERCIALIZED PHOTOAUTOTROPHIC MICROPROPAGATION SYSTEM USING LARGE VESSELS WITH FORCED VENTILATION: PLANT GROWTH AND ECONOMIC BENEFITS

Articles

A COMMERCIALIZED PHOTOAUTOTROPHIC MICROPROPAGATION SYSTEM USING LARGE VESSELS WITH FORCED VENTILATION: PLANT GROWTH AND ECONOMIC BENEFITS

Article number

725_35

Pages

279 – 292

Language

English

Abstract

A photoautotrophic or sugar-free medium micropropagation system (PAM) using five large culture vessels (volume: 120 L each) with a forced ventilation unit for supplying CO₂-enriched air was developed and applied to commercial production of calla lily (Zantedeschia elliottiana) and China fir (Cunninghamia lanceolata) plantlets.
The culture period of calla lily plantlets in the PAM was reduced by 50%, compared with that in a conventional, photomixotrophic micropropagation system (PMM) using small vessels each containing a sugar-containing medium.
Percent survival ex vitro of calla lily plantlets from the PAM was 95%, while that from the PMM was 60%. The production cost of calla lily in the PAM was reduced by about 40%, compared with that in the PMM, and the initial investment per plantlet for the PAM was about 10% lower than that for the PMM. The sales price of ex vitro acclimatized calla lily plantlet was increased by 25% due to its higher quality, compared with plantlets produced in the PMM.

Publication

V International Symposium on In Vitro Culture and Horticultural Breeding

Authors

T. Kozai, Q.T. Nguyen, Y. Xiao

Keywords

CO₂ enrichment, Cunninghamia lanceolata, sugar-free culture, Zantedeschia elliottiana

Full text

https://doi.org/10.17660/ActaHortic.2006.725.35

Online Articles (120)

1. PLANT BIOTECHNOLOGY AND ITS APPLICATION IN HORTICULTURE IN CHINA

Z.H. X

2. PLANT STRESS IN VITRO: THE ROLE OF PHYTOHORMONES

J. van Staden | C.W. Fennell | N.J. Taylor

3. HORTICULTURAL BIOTECHNOLOGY IN BRAZIL

M.G. Cardoso Costa | A. Xavier | W. Campos Otoni

4. PLANT BIOTECHNOLOGY IN TURKEY

H.A. Öktem

5. SOME ASPECTS OF EMBRYO DEVELOPMENT IN VITRO

A. Pret´ova | B. Obert

6. THE APPLICATION OF BIOTECHNOLOGY IN AN INTEGRATED PROJECT OF CONSERVATION AND UTILIZATION OF PAPAYA AND ITS WILD RELATIVES

S.E. Ashmore | R.A. Drew

7. REGENERATION-PROMOTING GENES IMPROVE TRANSFORMATION EFFICIENCY IN APRICOT

S. Lopez-Noguera | C. Petri | E. Olmos | L. Burgos

8. THE PROGRAMMED CELL DEATH, RESCUING CULTURE AND POLYPLOID PLANTLET ESTABLISHMENT IN ENDOSPERM CULTURE OF SPIDER LILIES (LYCORIS SPP.)

L.S. Huang | P.C. Kuo | C.T. Shii

9. IN VITRO FLOWERING OF SPATHIPHYLLUM CANNIFOLIUM: INFLUENCES OF GIBBERELLIC ACID, CULTURE TYPE AND SUCROSE CONCENTRATION

Y.H. Dewir | E.J. Hahn | K.Y. Paek

10. INTERSPECIFIC HYBRIDIZATION IN WOODY ORNAMENTALS: HOW TO DEAL WITH BARRIERS?

T. Eeckhaut | J. Van Huylenbroeck | K. Van Laere | E. Van Bockstaele

11. ENHANCED REGENERATIVE CAPACITY IN RHIPSALIDOPSIS AND SCHLUMBERGERA EXPLANTS BY IN VITRO MANIPULATIONS

S. Sriskandarajah | E. Al-Ramamneh | M. Serek

12. MOLECULAR FARMING AND ANTIBODY EXPRESSION IN DIFFERENT CELL COMPARTMENTS IN HIGHER PLANTS

13. CHANGES IN PHOSPHOLIPID CONTENT OF NUCLEAR SUBFRACTIONS DURING GERMINATION OF WHEAT SEEDLINGS UNDER INFLUENCE OF GIBBERELLIC ACID

L.A. Minasbekyan | ZH.V Yavroyan | M.R. Darbinyan | P.O. Vardevanyan

14. IN VITRO PROPAGATION OF CAPPARIS SPINOSA

E. Horshati | E. Jambor-Benczur

15. IN VITRO MORPHOGENESIS OF SHOOTS FROM LEAF EXPLANTS OF CUCUMBER (CUCUMIS SATIVUS L.)

N. Selvarai | A. Ganapath | A. Vasudevan | G. Vengadesan | S. Kasthuri Rengan

16. INCREASE IN THE QUALITY OF RASPBERRY CULTURES UNDER THE INFLUENCE OF FEEDDHA

M. Zawadzka | T. Orlikowska

17. IN VITRO REGENERATION OF ALLIUM AFLATUNENSE BY SOMATIC EMBRYOGENESIS

A. Subotic | S. Jevremonic | M. Trifunovic | LJ. Radojevic

18. IN VITRO PLANT REGENERATION IN ZYGOTIC EMBRYO CULTURE OF IRIS REICHENBACHII

S. Jevremonic | A. Subotic | LJ. Radojevic

19. AGAR PHENOLIC IMPURITIES AFFECT THE BIOLOGICAL PERFORMANCE OF IN VITRO CULTURES

M. Beruto | P. Curir | C. Portogallo

20. IMPACT OF SODIUM-SELENATE ON THE GROWTH OF RADISH SEEDLINGS IN VITRO

21. PROTOPLAST CULTURE IN PETUNIA HYBRIDA AND EFFECTS OF CELL CYCLE INHIBITORS ON CELL DIVISION FREQUENCY

L. Meyer | M. Serek | T. Winkelmann

22. HOW CAN DIFFERENT CYTOKININS INFLUENCE THE PROCESS OF SHOOT REGENERATION FROM APPLE LEAVES IN ´ROYAL GALA´ AND ´M.26´

23. EFFECTS OF CARBON AND NITROGEN SOURCES ON PROTOCORM-LIKE BODIES PROLIFERATION OF DENDROBIUM HUOSHANENSE BY LIQUID CULTURE

J.P. Luo | X.Q. Zha | X.J. Fan

24. ANALYSIS OF SUITABILITY OF DNA EXTRACTION PROTOCOLS FOR SOMACLONAL VARIATION ANALYSIS IN IN VITRO-CULTURED ORCHIDS

J.A. Teixeira da Silva | M. Tanaka

25. BREEDING OF ROSA RUGOSA THUNB. USING DIFFERENT TISSUE CULTURE METHODS

G. Jakobsone | D. Rieksta | A. Rihtere

26. INFLUENCE OF ABA ON STORAGE POSSIBILITIES IN VITRO OF LILACS

G. Jakobsone | D. Auzenbaha | D. Megre

27. MORPHOGENESIS IN FRAGARIA TISSUE CULTURE AFTER BIOLISTIC TREATMENT WITH NITROGEN FIXING BACTERIA

E. Preininger | Z. Kristof | J. Zatyko | ZS. Lorincz | I. Gyurjan

28. A COMPARATIVE HISTOLOGICAL STUDY BETWEEN ´EMBRYOGENIC STRUCTURES´ AND ZYGOTIC EMBRYOS OF CLEMATIS HYBRIDS CULTIVATED IN VITRO

L. Arène | M. Chevalier | N. Cognée | M. Tellier | A. Cadic

29. HISTOLOGY OF ADVENTITIOUS SHOOT PRODUCTION IN MICROPROPAGATED WALKING STICK CABBAGE (BRASSICA OLERACEA VAR. LONGATA)

Y. Zhao | J. Green | M. Yu | B.W.W Grout

30. IN VITRO EMBRYO RESCUE OF INTERSPECIFIC CROSSES FOR TRANSFERRING VIRUS RESISTANCE IN OKRA (ABELMOSCHUS ESCULENTUS (L.) MOENCH)

L. Rajamony | M. Chandran | K. Rajmohan

31. EMERGING THEMES IN IN VITRO CULTURE AND THEIR APPLICATIONS TO HORTICULTURE

A. Taji | P. Lakshmanan | R. Williams

32. ANEUPLOIDY, A SOURCE OF GENETIC DIVERSITY FOR FRUIT SPECIES

PH. Druart

33. NEW AROMATIC CYTOKININS CAN MAKE THE DIFFERENCE

I. Bogaert | S. Van Cauter | S.P.O. Werbrouck | K. Dolezal

34. EFFECT OF SUBSTRATE, AUXIN SOURCE AND AUXIN OVERPRODUCING MUTANTS OF ECTOMYCORRHIZA HEBELOMA CYLINDROSPORUM ROMAGNESI IN ROOTING OF TWO QUINCE CLONES (EM A AND BA 29) MICROSHOOTS

P. Pavlidis | E. Barbas | M. Vasilakakis

35. A COMMERCIALIZED PHOTOAUTOTROPHIC MICROPROPAGATION SYSTEM USING LARGE VESSELS WITH FORCED VENTILATION: PLANT GROWTH AND ECONOMIC BENEFITS

T. Kozai | Q.T. Nguyen | Y. Xiao

36. MICROPROPAGATION INDUSTRY IN INDIA: BIOLOGY AND BUSINESS

J. Prakash

37. HEALTHY IN VITRO PROPAGATION BY MERISTEM TIP CULTURE OF HELLEBORUS NIGER¿S SELECTED CLONE FOR CUT FLOWER

R. Poupet | L. Cardin | A. Henri | J.P. Onesto

38. BIOTECHNOLOGIES FOR THE PRESERVATION OF SELECTED RED CHICORY (CICHORIUM INTYBUS L.) LINES

39. ELIMINATION AND DETECTION OF PATHOGENS FROM TISSUE CULTURES OF PRUNUS SP.

40. GROWTH AND PHYSIOLOGICAL CHARACTERISTICS OF PLANTLETS OF SPATHIPHYLLUM ´SENSATION´ IN ROOTING PHASE AS AFFECTED BY SUCROSE-FREE MEDIUM AND DIFFUSIVE VENTILATION

F.X. Liao | B.Q. Wang | F.N. Xu | Z.Y. Yang | G.P. Wang

41. IN VITRO GERMINATION OF THREE OLIVE CULTIVARS

M. Kiani | Z. Zamani | A. Ebadi

42. SOMACLONAL VARIATION INDUCED DE NOVO LEAF CHIMERIC MUTANTS DURING IN VITRO PROPAGATION OF AFRICAN VIOLET (SAINTPAULIA IONANTHA WENDL.)

K. Shaijee | A. Tehranifar | R. Naderi | A. Khalighi

43. MICROPROPAGATION OF STEMONA CURTISII HOOK F., A THAI MEDICINAL PLANT

N. Montri | CH. Wawrosch | B. Kopp

44. THE INFLUENCE OF LIGHT COLOUR ON MICROPROPAGATION OF CHRYSANTHEMUM

N. Miler | M. Zalewska

45. CRYOPRESERVATION OF ROSA SHOOT TIPS: IMPORTANCE OF PRECULTURE CONDITIONS

A. Halmagvi | I. Pinker

46. CONSERVATIVE MICROPROPAGATION OF SOME ENDEMIC OR RARE SPECIES FROM THE DIANTHUS GENUS

V. Cristea | M. Puscas | M. Miclaus | C. Deliu

47. MICROPROPAGATION OF HORSERADISH (ARMORACIA RUSTICANA)

A. Pawelczak | A. Majewska | A. Geszprych | B. Dabrowska

48. APPLICATION OF SILICON RUBBER GRAFTING CLIPS FOR IN VITRO PHYTOPATHOLOGICAL STUDIES

E.K. Toth | E. Kriston | G. Csillery | M.E. Csanyi

49. PROTOCORM-LIKE BODY FORMATION AND PLANT REGENERATION OF DENDROBIUM HUOSHANENSE, AN ENDANGERED MEDICINAL PLANT

Y. Wang | J.P. Luo | X.Q. Zha

50. THE EFFECT OF INHERITING FACTOR ON MINERAL NUTRITION OF LOW VIGOROUS SWEET CHERRY ROOTSTOCKS

DJ.V. Ruzic | R.M. Cerovic | LJ. Culafic

51. CRYOPRESERVATION OF EMBRYOGENIC SUSPENSION CULTURES OF CYCLAMEN PERSICUM MILL.

V. Mubmann | M. Serek | T. Winkelmann

52. PLANT REGENERATION FROM DIFFERENT EXPLANT TYPES OF STREPTOCARPUS SPEC. IN VITRO

R. Afkhami-Sarvestani | M. Serek | T. Winkelmann

53. MORPHOLOGICAL VARIATION OF CORMS IN PLANTS REGENERATED FROM CALLUSES OF TARO (COLOCASIA ESCULENTA SCHOTT)

K. Murakami | K. Takashi | K. Ogawa

54. IN VITRO PROPAGATION AND MULTIPLE SHOOT FORMATION FROM COTYLEDONARY NODES OF PISTACHIO

Y. Ozden-Tokatli | E.A. Ozudogru | A. Akcin

55. EFFECT OF MANNITOL AND ABA ON INDUCTION OF SOMATIC EMBRYOGENESIS IN TEA (CAMELLIA SINENSIS L.)

E.A. Ozudogru | Y. Ozden-Tokatli | A. Akcin

56. EFFECT OF FRUIT CRYOPRESERVATION ON IN VITRO GERMINATION OF ZYGOTIC EMBRYOS OF SEVERAL SPECIES OF ILEX

L.A. Mroginski | P.A. Sansberro | A.M. Scocchi | C.V. Luna | H.Y. Rey

57. STIMULATION OF IN VITRO BULBLET GROWTH IN GALANTHUS SPECIES WITH SUCROSE AND ACTIVATED CHARCOAL

I. Staikidou | C. Selby | G. Hanks

58. IN VITRO PROPAGATION OF DIANTHUS BALBISII SER. SUBSP. LIBURNICUS (BARTL.) PIGN. BY SHOOT TIP CULTURE

G. Berardi | R. Roncasaglia | D. Scaravelli | G. Dradi

59. OPTIMIZATION OF ROOTING OF IN VITRO PROPAGATED MALUS X DOMESTICA ¿PINOVA¿

J. Kolozsvari Nagy | S. Sule

60. THE INFLUENCE OF GROWTH RETARDANTS AND BA ON THE GROWTH AND DEVELOPMENT OF TIBOUCHINA URVILLEANA COGN. IN VITRO

D. Kozak

61. RESULTS WITH THE MICROPROPAGATION OF GALANTHUS ELWESII AND GALANTHUS NIVALIS ´FLORE PLENO´

A. Tilly-Mandy | E. Jambor-Benczur | J. Szabo

62. IN VITRO METHODS FOR TESTING POTATO CLONES AGAINST SOFT ROT ERWINIAE

I. Hudak | J. Dobranszki | M. Hevesi

63. INTERSPECIFIC HYBRIDIZATION IN GENUS FRAGARIA AND EVALUATION OF THE HYBRIDS FOR COLD HARDINESS AND RED STELE RESISTANCE USING IN VITRO METHODS

R. Rugienius | D. Gelvonauskiene | I. Zalunskaite | J. Pakalniskyte | V. Stanys

64. MICROPROPAGATION OF ´ECHO´ CULTIVARS OF EUSTOMA GRANDIFLORUM

M. Ordogh | E. Jambor-Benczur | A. Tilly-Mandy

65. EFFECT OF PROPAGATION METHOD ON PLANT DEVELOPMENT AND CONTENT OF BIOLOGICALLY ACTIVE COMPOUNDS IN THE HERB OF ST. JOHN´S WORT (HYPERICUM PERFORATUM)

A. Pawelczak | E. Osinska | A. Geszprych | Z. Weglarz

66. PROLIFERATION AND COLD HARDENING OF IN VITRO GROWN APPLE SHOOT TIPS

J. Sedlak | F. Paprstein | A. Bilavcik | J. Zamecnik

67. EFFECTS OF TEMPERATURE ON THE GROWTH AND DORMANCY OF TISSUE-CULTURED HERBACEOUS PEONY SHOOTS

E. Gabryszewska

68. IN VITRO MORPHOGENESIS IN ZYGOTIC EMBRYO CULTURES OF CAROB TREE (CERATONIA SILIQUA L.)

L. Custodio | A. Romano

69. IN VITRO SLOW GROWTH STORAGE OF FRUIT ROOTSTOCKS INSIDE GAS-TIGHT OR GAS-PERMEABLE CONTAINERS

70. AN ALTERNATIVE PATHWAY FOR PLANT IN VITRO REGENERATION OF INSECTICIDAL TREE MELIA AZEDARACH L. DERIVED FROM THE INDUCTION OF SOMATIC EMBRYOGENESIS

71. MICROPROPAGATION, DETECTION AND ELIMINATION OF DMV IN THE CZECH COLLECTION OF DAHLIA

J. Sediva | P. Novak | J. Kanka | J. Laxa

72. THE EFFECT OF BION® (BENZOTHIADIAZOLE) ON MULTIPLICATION AND ROOTING OF HOSTA CULTIVARS

73. LONG TERM SURVIVAL OF CRYPTIC VIRUSES IN ASEPTICALLY GROWN IN VITRO PROPAGATED PLANTS

A. Szego | P. Ilyes | E.K. Toth | L. Potvondi | N. Lukacs

74. MICROPROPAGATION OF PEACH ROOTSTOCKS AND CULTIVARS

I. Balla | Z. Kirilla

75. EFFECT OF CULTURE VESSELS ON THE BIOMASS PRODUCTION AND TOTAL ANTIOXIDANT CAPACITY IN PEA SEEDLINGS GERMINATED UNDER PAM-SYSTEM

76. OPTIMIZATION OF TISSUE CULTURE MEDIA FOR PROPAGATION OF MARIANNA ROOTSTOCKS

E.L. Mansvelt | L. Watts | M.H. Spreeth | A. Meszaros

77. BIOTECHNOLOGY OF ANNUAL FLOWER PLANTS: MICROPROPAGATION OF RUDBECKIA SP.

P. Szarvas | A. Zsila-André | M.G. Fari | Z. Kovats

78. COST EXPERIENCES WITH BIOREACTORS

A.K. Hyoslef-Eide | B. Ruffoni | K. Saare-Surminski | W. Preil

79. DESIGN OF A REVERT ROTARY PLANT MICROPROPAGATION BIOREACTOR ¿ ´3R´ SYSTEM: APPLICATION FOR RESEARCH, POSSIBILITIES OF SCALING-UP AND LIMITATIONS

M.G. Fari | T. Kertesz | M. Laszlo | ZS. Varga

80. MASS PROPAGATION OF COFFEE TRANSPLANTS UNDER SCALED-UP PHOTOAUTOTROPHIC MICROPROPAGATION SYSTEM

F. Afreen | S.M.A. Zobayed | T. Kozai

81. MASS PROPAGATION OF CHERRY (CERASUS × YEDOENSIS MATSUM.) THROUGH SHOOT PRIMORDIA

82. APPLICATION OF TISSUE CULTURE AND MOLECULAR FARMING IN THE PRODUCTION OF PHARMACEUTICALS

S. Sorvari | M.G. Fari | M. Laszlo | O. Toldi

83. APPLICATION OF THE TEMPORARY IMMERSION SYSTEM FOR THE IN VITRO PRODUCTION OF BIOACTIVE COMPOUNDS IN HARPAGOPHYTUM (DEVIL’S CLAW)

84. COMPARISON OF GROWTH AND SECONDARY METABOLITE ACCUMULATION IN CULTURES OF COMPACT CALLUS AGGREGATES AND SHOOTS OF HYPERICUM PERFORATUM L. IN SHAKE FLASKS AND IN A BUBBLE COLUMN BIOREACTOR

85. ENHANCING THE BIOTRANSFORMATION RATE OF RHODIOLA ROSEA CALLUS CULTURES

Z. Gyorgy | P. Neubauer | A. Tolonen | A. Hohtola

86. AN EFFICIENT MICROPROPAGATION SYSTEM: 3R BIOREACTOR

A. Mészaros | E. Domokos-Szabolcsy | K. Halasz | K. Kalai | M.G. Fari

87. APPLICATION OF SCAR MARKERS LINKED TO A PUTATIVE PRSV-P RESISTANCE LOCUS FOR ASSISTED BREEDING OF RESISTANT C. PAPAYA CULTIVARS

S. Dillon | C. Ramage | M. O'Brien | R.A. Drew

88. IDENTIFICATION OF GENES INVOLVED IN RIPENING OF OCTOPLOID STRAWBERRY (FRAGARIA X ANANASSA DUCH.) USING CDNA-AFLP

A. Balogh | E. Kiss | T. Koncz | L. Heszky

89. GAMMA RAYS INDUCED IN VITRO MUTAGENESIS AND MOLECULAR MARKER-ASSISTED SELECTION OF MUTANTS IN GRAPEVINE

R.N. Khawale | S.K. Singh | Y. Vimala

90. IN VITRO CULTURE AND GENETIC ANALYSIS OF MALE AND FEMALE DATE PALM (PHOENIX DACTYLIFERA L.)

N.S. Al-Khalifah | F.A. Khan | E. Askari | S. Hadi

91. NIR – AN ALTERNATIVE TOOL IN HORTICULTURAL BIOTECHNOLOGY

92. USING SSR MARKERS TO DISTINGUISH APPLE CULTIVARS

Z. Galli | G. Halasz | E. Kiss | J. Dobranszki | L. Heszky

93. AGGRESSIVENESS OF PHYTOPHTHORA INFESTANS ISOLATES CORRELATES WITH THEIR PROTEOLYTIC ACTIVITY

J.A. Hamill | C. Selby | L.R. Cooke

94. IMPROVEMENT OF BULB FORMATION IN MICROPROPAGATED TULIPS BY TREATMENT WITH NAA AND PACLOBUTRAZOL OR ANCYMIDOL

M. Podwyszynska

95. POTENTIAL RAPD APRICOT MARKER LINKED TO PPV RESISTANCE

M. Vachun | M. Kafonkova | M. Pidra | J. Raddova | B. Krska

96. CHARACTERIZATION OF DIFFERENT APRICOT CULTIVARS GROWN IN HUNGARY WITH SSR MARKERS

A. Pedryc | SZ. Ruthner | GY.D. Bisztray | M. Laimer

97. GENOTYPE IDENTIFICATION OF ONION (ALLIUM CEPA L.) CULTIVARS

98. DISTINGUISHING OF THE GRAPEVINE CULTIVARS ´PICOLIT´ AND ´KÉKNYELŰ´ WITH THE HELP OF IZOENZYME ANALYSES

G. Györffyné Jahnke

99. NIR SPECTROSCOPY FOR DISTINCTION OF HORTICULTURAL PLANT SEEDS

GY.D. Bisztray | T. Deak | ZS. Seregély | K. Kaffka

100. MORPHOLOGICAL AND GENETIC INVESTIGATION OF MEDIAEVAL GRAPE SEEDS

101. PATENTS AND TRANSGENIC PLANTS

J.M. Dunwell

102. A REAL THREAT OF MANIPULATION

E. Balazs

103. AGROBACTERIUM-MEDIATED TRANSFORMATION OF PELARGONIUM (PELARGONIUM ZONALE HYBRIDS AND PELARGONIUM PELTATUM HYBRIDS)

T. Winkelmann | K. Kayiani | M. Serek

104. TRANSFORMATION OF WOODY SPECIES: STATE OF THE ART

105. PHYTOREMEDIATION WITH TRANSGENIC PLANTS

106. A SELECTION STRATEGY TO OBTAIN TRANSFORMED APRICOT PLANTS

C. Petri | S. Lopez-Noguera | L. Burgos | N. Alburquerque

107. ASSESSMENT OF REGENERATION AND TRANSFORMATION IN SWEET CHERRY CULTIVARS

M.C. Piagnani | A. Scotti

108. TRICHODERMA CHITINASE GENE EXPRESSION CONFERS MOULD RESISTANCE

K. Kalai | G. Giczey | A. Mészaros | F. Dénes | E. Balazs

109. EXPERIMENTS ON TRANSFORMATION AND IN VITRO REGENERATION OF SQUASH VARIETIES

110. TOWARDS AGROBACTERIUM-MEDIATED TRANSFORMATION OF HIPPEASTRUM X CHMIELII CHM. ¿ IDENTIFICATION OF APPROPRIATE SELECTION MEDIA

A. Ilczuk | A. Lukaszewska | T. Winkelmann | M. Serek

111. ELECTROFUSION OF PROTOPLASTS IN SELECTED VEGETABLES OF BRASSICA, CUCUMIS AND SOLANUM GENERA

112. EFFECTS OF MODIFIED CARBOHYDRATE CONTENT ON THE DEVELOPMENT AND GROWTH OF CARNATION (DIANTHUS CARYOPHYLLUS L.)

A. Szoke | E. Kiss | I. Kerepesi | O. Toldi | L. Heszky

113. TRANSFORMATION OF APPLE (MALUS DOMESTICA BORKH.) USING THE PHOSPHOMANNOSE ISOMERASE GENE AS A SELECTABLE MARKER

J. Degenhardt | I. Szankowski

114. HAPLOID PRODUCTION AND CHROMOSOME DOUBLING

K.J. Kasha | Y.S. Shim | E. Simion | J. Letarte

115. DEVELOPMENT OF DOUBLE HAPLOIDY IN UMBELLIFERAE

A.M.R. Ferrie | T.D. Bethune | D. Watere

116. CUCUMBER (CUCUMIS SATIVUS L.) DIHAPLOID LINE PRODUCTION USING IN VITRO RESCUE OF IN VIVO INDUCED PARTHENOGENIC EMBRYOS

R. Dolcet-Sanjuan | E. Claveria | J. Garcia-Mas

117. PRODUCTION OF DOUBLED HAPLOID BREEDING LINES IN CASE OF PAPRIKA, SPICE PAPRIKA, EGGPLANT, CUCUMBER, ZUCCHINI AND ONION

118. ANDROGENESIS INDUCTION FROM TOMATO ANTHER CULTURES: CALLUS CHARACTERIZATION

J.M. Segui-Simarro | F. Nuez

119. CRYOPRESERVATION OF POLLEN OF CAROB TREE

L. Custodio | N. Fernandes | M.F. Carneiro | A. Romano

120. FLAX PLANTS REGENERATED FROM UNPOLLINATED OVULES CULTURED IN OVARY SEGMENTS

Z. Bartosova | S. Masar | A. Pretova