Home > V International Symposium on In Vitro Culture and Horticultural Breeding > IN VITRO MORPHOGENESIS IN ZYGOTIC EMBRYO CULTURES OF CAROB TREE (CERATONIA SILIQUA L.)

Articles

IN VITRO MORPHOGENESIS IN ZYGOTIC EMBRYO CULTURES OF CAROB TREE (CERATONIA SILIQUA L.)

Article number

725_68

Pages

477 – 482

Language

English

Abstract

In this study, organogenic cultures were induced from zygotic embryos of carob tree.
Basal medium consisted of Murashige & Tucker (1969), supplemented with 0.5 or 1 mg L^-1 of 2,4-dichlorophenoxyacetic acid alone or combined with 0.5, 1, 2, 3 and 4 mg L^-1 of kinetin.
Calli initiation occurred in all the hormonal combinations tested.
The zygotic embryo cultures differentiated two types of regenerants: somatic embryos (SEs) and shoots, with a maximum frequency of 30 and 100%, respectively.
SEs exhibited morphological abnormalities, such as fusion and absence of cotyledons, and showed secondary embryogenesis.
The regenerated shoots were rooted, hardened and transferred to the soil.

Publication

V International Symposium on In Vitro Culture and Horticultural Breeding

Authors

L. Custodio, A. Romano

Keywords

conversion, regeneration, organogenesis, somatic embryogenesis, tissue culture

Full text

https://doi.org/10.17660/ActaHortic.2006.725.68

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