Articles
APPLICATION OF SILICON RUBBER GRAFTING CLIPS FOR IN VITRO PHYTOPATHOLOGICAL STUDIES
Article number
725_48
Pages
373 – 378
Language
English
Abstract
Plant grafting is a tool for reducing plant pathological problems in case of many horticultural crops.
Silicon rubber grafting clips are commonly used in melon and tomato production.
Their application is easy and very effective (near 100%). Silicon clip is autoclavable, so it can be used in phytopathological examinations in vitro as well (e.g. transmission tests of viruses, viroids and phytoplasmas, checking of resistance etc.).
Sterile in vitro plants with short roots were removed from the media and the stem of the scion and the stock (at the four to five-leaf stage) were cut at right angles, each with 2-3 leaves remaining on the stem.
The stem of the scion was cut in a wedge with scalpel in Petri-dish, and the tapered end was fitted into a cleft cut in the end of the stock.
The graft was firmed with silicon rubber clip hauled up with dentists forceps.
Finally the plants were placed into fresh media.
The survival rate of the grafted plant was excellent especially in case of herbaceous plants (90-100%), it was reasonable for woody plants (30%).
The efficiency of our in vitro grafting method was demonstrated by quick transmission of the model pathogens confirmed by reverse transcription and polymerase chain reaction (RT-PCR). Valuable experimental data could be achieved within less than two weeks.
Silicon rubber grafting clips are commonly used in melon and tomato production.
Their application is easy and very effective (near 100%). Silicon clip is autoclavable, so it can be used in phytopathological examinations in vitro as well (e.g. transmission tests of viruses, viroids and phytoplasmas, checking of resistance etc.).
Sterile in vitro plants with short roots were removed from the media and the stem of the scion and the stock (at the four to five-leaf stage) were cut at right angles, each with 2-3 leaves remaining on the stem.
The stem of the scion was cut in a wedge with scalpel in Petri-dish, and the tapered end was fitted into a cleft cut in the end of the stock.
The graft was firmed with silicon rubber clip hauled up with dentists forceps.
Finally the plants were placed into fresh media.
The survival rate of the grafted plant was excellent especially in case of herbaceous plants (90-100%), it was reasonable for woody plants (30%).
The efficiency of our in vitro grafting method was demonstrated by quick transmission of the model pathogens confirmed by reverse transcription and polymerase chain reaction (RT-PCR). Valuable experimental data could be achieved within less than two weeks.
Authors
E.K. Toth, E. Kriston, G. Csillery, M.E. Csanyi
Keywords
in vitro grafting, grafting clips, RT-PCR
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