Articles
SEED POTATOES PRODUCTION IN CYPRUS STARTING FROM IN VITRO CULTURE OF APICAL MERISTEM
Article number
741_35
Pages
283 – 288
Language
English
Abstract
Sixteen potato tubers from each of the cultivars Spunda, Nicola, and Cara were incubated for thermotherapy under 36°C and RH 90% for 40 days.
Subsequently from each of these tubers meristems 0.5 mm long were isolated and cultured in-vitro on a modified MS (Murashige and Skoog, 1962) medium and micro-plants were produced.
The medium contained MS basic salts and vitamins, supplemented with 3% sucrose, 4.5 mg/L BAP, 0.009 mg/L IBA, 55.7 mg/L ascorbic acid and was solidified with 2.5 g/L phytagel.
The potato tubers were planted and tested by ELISA for viruses and other pathogens of seed potato quarantine value.
All infected cultures resulted from infected tubers were discarded.
In vitro potato plant material was tested when it was necessary with the molecular method ds-RNA and RT-PCR for viruses and viroids.
Plantlets were produced and hardened in vitro by increasing the light intensity to 4000 lux for two weeks and ex vitro in additional light and in a micro fog at relevant humidity (RH) progressively reduced from 95% to 55%. Plantlets were also hardened in growth chambers.
Subsequently, plantlets were planted in pots in an insect proof glasshouse for production of mini-tubers.
Mini tubers production was continue for three generations and many parameters were measured for seed quality and growth emergence of the tubers.
After completing the final tests, seed potatoes were given to growers for one generation multiplication to produce the final seed potatoes for the potato grower.
Subsequently from each of these tubers meristems 0.5 mm long were isolated and cultured in-vitro on a modified MS (Murashige and Skoog, 1962) medium and micro-plants were produced.
The medium contained MS basic salts and vitamins, supplemented with 3% sucrose, 4.5 mg/L BAP, 0.009 mg/L IBA, 55.7 mg/L ascorbic acid and was solidified with 2.5 g/L phytagel.
The potato tubers were planted and tested by ELISA for viruses and other pathogens of seed potato quarantine value.
All infected cultures resulted from infected tubers were discarded.
In vitro potato plant material was tested when it was necessary with the molecular method ds-RNA and RT-PCR for viruses and viroids.
Plantlets were produced and hardened in vitro by increasing the light intensity to 4000 lux for two weeks and ex vitro in additional light and in a micro fog at relevant humidity (RH) progressively reduced from 95% to 55%. Plantlets were also hardened in growth chambers.
Subsequently, plantlets were planted in pots in an insect proof glasshouse for production of mini-tubers.
Mini tubers production was continue for three generations and many parameters were measured for seed quality and growth emergence of the tubers.
After completing the final tests, seed potatoes were given to growers for one generation multiplication to produce the final seed potatoes for the potato grower.
Publication
Authors
G.J. Minas, S. Gregoriou, TH. Kapari-Isaia, L. Papayiannis
Keywords
seed potatoes, meristem culture, in-vitro, mini-tubers, ‘Spunda’, ‘Nicola’, ‘Cara’
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