Articles
A PROTOCOL FOR IN VITRO RAPID MICRO PROPAGATION OF FRENCH TARRAGON STARTING FROM APICAL MERISTEM
Article number
741_37
Pages
295 – 300
Language
English
Abstract
Optimum proliferation and production of micro plants of the medicinal and aromatic French tarragon (Artemisia dranunculus L. var. sativa) in vitro was obtained by culturing apical meristem-tips 0.5 mm long on modified MS (Murashige and Skoog, 1962) medium.
The medium contained MS basic salts and vitamins, supplemented with 3% sucrose, 4.5 mg/L BAP, 0.009 mg/L IBA, 55.7 mg/L ascorbic acid and was solidified with 2.5 g/L phytagel.
After planting of meristem tip cultures were incubated in the deep dark for the first two weeks to totally prevent the blackening of the tissues and halo formation, which facilitated the optimum initiation of the cultures.
Cultures were transferred to a growth room with 16 to 8 hours day night at light intensity 1000 lux and 21 ±2°C. Proliferation was on an average 3-fold per 2 weeks and progressively increasing as cultures matured.
Plantlets produced were undergoing two forms of hardening one in vitro by increasing the light intensity to 2000 lux for two weeks and one ex vitro in a micro fog at relevant humidity (RH) progressively reduced from 95% to 55%. After these treatments plantlets were established well when transferred to glasshouse growing conditions.
Using this method, considerable numbers of rooted in vitro microplants free of visible genetic aberrations were produced after six months of culture initiation.
This method is used for production of pathogen free and genetically uniform microplants for immediate release to the growers.
The medium contained MS basic salts and vitamins, supplemented with 3% sucrose, 4.5 mg/L BAP, 0.009 mg/L IBA, 55.7 mg/L ascorbic acid and was solidified with 2.5 g/L phytagel.
After planting of meristem tip cultures were incubated in the deep dark for the first two weeks to totally prevent the blackening of the tissues and halo formation, which facilitated the optimum initiation of the cultures.
Cultures were transferred to a growth room with 16 to 8 hours day night at light intensity 1000 lux and 21 ±2°C. Proliferation was on an average 3-fold per 2 weeks and progressively increasing as cultures matured.
Plantlets produced were undergoing two forms of hardening one in vitro by increasing the light intensity to 2000 lux for two weeks and one ex vitro in a micro fog at relevant humidity (RH) progressively reduced from 95% to 55%. After these treatments plantlets were established well when transferred to glasshouse growing conditions.
Using this method, considerable numbers of rooted in vitro microplants free of visible genetic aberrations were produced after six months of culture initiation.
This method is used for production of pathogen free and genetically uniform microplants for immediate release to the growers.
Publication
Authors
G.J. Minas
Keywords
French tarragon, in vitro, meristem culture, mass-micropropagation
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