Articles
SANITATION OF LOCAL CITRUS VARIETIES AND / OR CLONES IN CYPRUS
Article number
741_38
Pages
301 – 306
Language
English
Abstract
The micrografting in vitro technique was used in Cyprus during 2000-2005 to free local citrus cultivars and/or clones from virus and other pathogens within the context of a special programme named « ESPERIDES ». A survey in commercial groves was conducted for selection of elite mother plants of citrus, including Polyphori and Lapithou lemon (Citrus limon Burm f.), Jaffa, Siekeriko and Aematoysiki orange (C. sinensis L.), Arakapas mandarin (C. reticulata Blanco), Frappa (C. grandis (L.) Osbeck), Bergamot (C. bergamia Risso & Poit.), Coumantantas (C. coumantatore), and Pummelo (C. maxima). All mother plants were tested for viruses and viroids by biological indexing, by ELISA for CTV, and by RT-PCR for viroids.
All mother trees were found free of CTV, CVV, concave gum and impietratura, but were infected by CEVd and/or other viroids, including CCaVd and CBLVd.
Some mother trees were infected by CPsV. The standard procedure of shoot-tip grafting technique was used for elimination of viroids and CPsV in the selected citrus isolates.
Average micro grafting success was 40-60% from the 500 micro grafts.
Micro grafted plants, re-grafted on sour orange seedlings in vivo, and successfully established and transferred to the glasshouse, were tested 6-9 months later for the viruses and viroids present in meristem donor mother plants.
Three Polyphori lemon, six Arakapas mandarin, twenty Jaffa orange plants, three Bergamot, two Frappa and one Coumantantas produced by micro grafting from different source trees were found free of all viroids and CPsV and were used as primary source material for the basic citrus plantation of the island and for further propagation to government mother stock plantations, private nurseries and growers.
Tests for viroids and CPsV are being continued for all plants which were produced by shoot-tip grafting in vitro.
All mother trees were found free of CTV, CVV, concave gum and impietratura, but were infected by CEVd and/or other viroids, including CCaVd and CBLVd.
Some mother trees were infected by CPsV. The standard procedure of shoot-tip grafting technique was used for elimination of viroids and CPsV in the selected citrus isolates.
Average micro grafting success was 40-60% from the 500 micro grafts.
Micro grafted plants, re-grafted on sour orange seedlings in vivo, and successfully established and transferred to the glasshouse, were tested 6-9 months later for the viruses and viroids present in meristem donor mother plants.
Three Polyphori lemon, six Arakapas mandarin, twenty Jaffa orange plants, three Bergamot, two Frappa and one Coumantantas produced by micro grafting from different source trees were found free of all viroids and CPsV and were used as primary source material for the basic citrus plantation of the island and for further propagation to government mother stock plantations, private nurseries and growers.
Tests for viroids and CPsV are being continued for all plants which were produced by shoot-tip grafting in vitro.
Publication
Authors
TH. Kapari-Isaia, A. Kyriakou, I. Ioannides, L. Papayiannis, G.T. Minas
Keywords
shoot-tip grafting, indexing, elimination of viruses, pre-basic plantation
Online Articles (38)