Articles
SPECIAL MICROGRAFTING TECHNIQUE FOR CHERRY (PRUNUS AVIUM L.)
Article number
764_18
Pages
151 – 154
Language
English
Abstract
The success of different in vitro micrografting techniques and scion size for cherry (Prunus avium Seeyahe Mashad) was studied.
Grafting success (union of grafts) was significantly dependent on the method of grafting.
The highest percentage (65%) of successful grafts was obtained with the homoplastic apex graft (shoot-tip), with apical bud scion length greater than 6 mm.
However, the success of heteroplastic side bud apices (wedge) grafts was only 16%. The homoplastic micrografting method in vitro can be a useful technique in the improvement, rejuvenation and freeing from viruses for fruit crops.
There was good efficiency and maintenance of good contact between scion and rootstock until fusion took place by using this technique.
In vitro germinated sour cherry seedlings (Prunus cerasus Albaloo) which emerged one month after inoculation, were decapitated and used as rootstocks.
One-month-old in vitro-cultured meristematic apices with length of 515 mm were used as microscions.
The highest amount of successful grafts was obtained with the homoplastic apex graft (shoot-tip), when compared with wedge grafts and heteroplastic grafts.
The graft union, as measured by graft compatibility, was satisfactory, although, it formed very slowly during the first month.
Contamination and necroses occurred more often under in vitro conditions.
Nutrient disorders and mineral deficiency symptoms were observed.
This technique has a good potential for mass propagation of disease-free sweet cherry.
Grafting success (union of grafts) was significantly dependent on the method of grafting.
The highest percentage (65%) of successful grafts was obtained with the homoplastic apex graft (shoot-tip), with apical bud scion length greater than 6 mm.
However, the success of heteroplastic side bud apices (wedge) grafts was only 16%. The homoplastic micrografting method in vitro can be a useful technique in the improvement, rejuvenation and freeing from viruses for fruit crops.
There was good efficiency and maintenance of good contact between scion and rootstock until fusion took place by using this technique.
In vitro germinated sour cherry seedlings (Prunus cerasus Albaloo) which emerged one month after inoculation, were decapitated and used as rootstocks.
One-month-old in vitro-cultured meristematic apices with length of 515 mm were used as microscions.
The highest amount of successful grafts was obtained with the homoplastic apex graft (shoot-tip), when compared with wedge grafts and heteroplastic grafts.
The graft union, as measured by graft compatibility, was satisfactory, although, it formed very slowly during the first month.
Contamination and necroses occurred more often under in vitro conditions.
Nutrient disorders and mineral deficiency symptoms were observed.
This technique has a good potential for mass propagation of disease-free sweet cherry.
Authors
M.E. Amiri
Keywords
cherry, graft union, heteroplastic graft, homoplastic graft, microscion
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