EFFECT OF COLD TREATMENT TIME ON STEM-DISC CULTURE OF GARLIC (ALLIUM SATIVUM L.)

In garlic tissue culture, cytokinin generally needs to be added in medium to increase seedling propagation coefficient.
However, in this study, appropriate cold treatment at 4°C was also found to have the same effect on garlic bulb propagation.
Using Chinese local garlic variety (‘Sanyuehuang’) as the material and LS as the medium, cold treatment at 4°C respectively for 0, 2, 4, 6, 8 and 10 weeks, we studied the propagation and bulbs development of garlic in stem-disc culture.
The results showed that cold treatment could enhance the propagation coefficient of garlic and accelerate garlic bulb development.
The best effects were found in cold treatment for 6 and 4 weeks, while the propagation coefficients were high, up to 7.34 and 3.85, respectively.
At the same time, the ratio of micro-bulbs development reached 89.6% and 54.7%, rooting number was 2.8 and 4.9 with the average weight of bulb 70~80 mg.
The germination ratios of micro-bulbs were correlative to their own weights, and the germination ratios would be 100% when bulb weights were over 50 mg.

XXVII International Horticultural Congress – IHC2006: International Symposium on Plant Biotechnology: From Bench to Commercialization

Z. Wu, Y.J. Long, Q. Zhang, P.F. Li

cold treatment, garlic, micro-bulbs, propagation coefficient, stem-disc

https://doi.org/10.17660/ActaHortic.2007.764.28