Articles
SHOOT REGENERATION AND GENETIC TRANSFORMATION OF REGENERATIVE CALLUS CULTURE IN DIANTHUS HYBRIDA ‘TELSTAR SCARLET’
Article number
764_20
Pages
165 – 168
Language
English
Abstract
Regenerative callus was established and was successfully applied to Agrobacterium-mediated genetic transformation.
The callus was isolated from the base of adventitious shoots on cotyledon explants of Dianthus hybrida cv. Telstar Scarlet cultured on MS media supplemented with 1 mg L-1 TDZ and 0.1 mg L-1 NAA. This callus culture was successfully applied to Agrobacterium-mediated genetic transformation.
Transformed shoots were obtained from inoculated calli selected on MS medium containing 1.0 mg L-1 TDZ, 0.1 mg L-1 NAA, 20 g L-1 sucrose, 2 g L-1 gellan gum and 2080 mg L-1 G-418 within 20 weeks from inoculation.
All transformants grew, flowered normally and produced seeds when crossed with other Dianthus species.
The callus was isolated from the base of adventitious shoots on cotyledon explants of Dianthus hybrida cv. Telstar Scarlet cultured on MS media supplemented with 1 mg L-1 TDZ and 0.1 mg L-1 NAA. This callus culture was successfully applied to Agrobacterium-mediated genetic transformation.
Transformed shoots were obtained from inoculated calli selected on MS medium containing 1.0 mg L-1 TDZ, 0.1 mg L-1 NAA, 20 g L-1 sucrose, 2 g L-1 gellan gum and 2080 mg L-1 G-418 within 20 weeks from inoculation.
All transformants grew, flowered normally and produced seeds when crossed with other Dianthus species.
Authors
C. Nontaswatsri, K. Uchiyama, S. Fukai
Keywords
Agrobacterium-mediated genetic transformation, Dianthus hybrida ¿Telstar Scarlet¿, hormones
Online Articles (44)