Articles
THE RELATIONSHIPS AMONG CYMBIDIUM SINENSE CULTIVARS BASED ON RAPD ANALYSIS
Article number
766_42
Pages
323 – 330
Language
English
Abstract
Cymbidium sinense, a popular ornamental, has a long history of cultivation in China.
Many cultivars have been selected and used; however, their genetic background was unknown.
The objective of this study was to determine their genetic relationships using randomly amplified polymorphic DNA (RAPD) markers.
Thirty-two cultivars which mainly originated from Guangdong, Fujian, and Taiwan provinces of China were screened for polymorphic RAPD markers.
Genomic DNA was extracted from green leaves of these cultivars.
Sixteen random decamer primers were selected for PCR amplification.
Among the 195 DNA bands obtained, 179 bands were polymorphic.
The average number of DNA bands amplified by each primer was 12.2. All cultivars could be identified based on RAPD markers when a number of primers were considered.
Based on dice coefficients, genetic similarity among the cultivars ranged from 0.600 to 0.870. The cultivars can be divided into two clusters based on the UPGMA dendrogram, and this division is in consistence with the morphological classification based on flower color.
Cultivars originated from the same area were clustered together based on the principal coordinates analysis.
It is suggested that RAPD markers may be used for cultivar identification and relationship analysis in C. sinense.
Many cultivars have been selected and used; however, their genetic background was unknown.
The objective of this study was to determine their genetic relationships using randomly amplified polymorphic DNA (RAPD) markers.
Thirty-two cultivars which mainly originated from Guangdong, Fujian, and Taiwan provinces of China were screened for polymorphic RAPD markers.
Genomic DNA was extracted from green leaves of these cultivars.
Sixteen random decamer primers were selected for PCR amplification.
Among the 195 DNA bands obtained, 179 bands were polymorphic.
The average number of DNA bands amplified by each primer was 12.2. All cultivars could be identified based on RAPD markers when a number of primers were considered.
Based on dice coefficients, genetic similarity among the cultivars ranged from 0.600 to 0.870. The cultivars can be divided into two clusters based on the UPGMA dendrogram, and this division is in consistence with the morphological classification based on flower color.
Cultivars originated from the same area were clustered together based on the principal coordinates analysis.
It is suggested that RAPD markers may be used for cultivar identification and relationship analysis in C. sinense.
Publication
Authors
Genfa Zhu, Dongmei Li, Qingsheng Ye, Zhenfei Guo
Keywords
Cymbidium sinense, RAPD, genetic relationship, cultivar identification
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