Articles
DETECTION OF GENETIC VARIATION BY RAPD AMONG CHRYSANTHEMUM PLANTLETS REGENERATED FROM IRRADIATED CALLI
Article number
766_54
Pages
413 – 420
Language
English
Abstract
Genetic variation of regenerated plantlets following in vitro mutation was detected via random amplified polymorphic DNA (RAPD) in chrysanthemum.
RAPD bands produced from eighteen 10-mer arbitrary primers were used to assess the genetic variation of plantlets regenerated from floret-derived calli treated with 0, 10, 15 and 20 Gy gamma rays.
These primers generated a total of 167 reproducible RAPD bands ranging in size from 0.3 to 2.0 kb, of which 61.7% were polymorphic and 38.3% monomorphic.
The number of bands per primer ranged from 4 to 14, with an average of 9.3. The further analysis of RAPD result showed that genetic variation of generated plantlet was proportional to the dosage of gamma ray, while the 15 and 20 Gy treatments were not significantly different, which is consistent with the common conception that genetic variation of radiomutants is usually proportional to the dosage of mutagen within a certain range.
Therefore, we believe that RAPD is a useful technique for the rapid and easy assessment of genetic variation of mutants and may become a potential tool for the quick selection of mutants with great genetic variation during early growth stages.
RAPD bands produced from eighteen 10-mer arbitrary primers were used to assess the genetic variation of plantlets regenerated from floret-derived calli treated with 0, 10, 15 and 20 Gy gamma rays.
These primers generated a total of 167 reproducible RAPD bands ranging in size from 0.3 to 2.0 kb, of which 61.7% were polymorphic and 38.3% monomorphic.
The number of bands per primer ranged from 4 to 14, with an average of 9.3. The further analysis of RAPD result showed that genetic variation of generated plantlet was proportional to the dosage of gamma ray, while the 15 and 20 Gy treatments were not significantly different, which is consistent with the common conception that genetic variation of radiomutants is usually proportional to the dosage of mutagen within a certain range.
Therefore, we believe that RAPD is a useful technique for the rapid and easy assessment of genetic variation of mutants and may become a potential tool for the quick selection of mutants with great genetic variation during early growth stages.
Publication
Authors
Nianjun Teng, Fadi Chen, Zhongchun Jiang, Weimin Fang, Tingting Chen
Keywords
Chrysanthemum morifolium, gamma rays, genetic variation, in vitro mutation, random amplified polymorphic DNA (RAPD)
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