Articles
EXPRESSION PROFILING OF TOMATO RESPONSE TO PYRENOCHAETA LYCOPERSICI INFECTION
Article number
789_35
Pages
257 – 262
Language
English
Abstract
Corky root, caused by Pyrenochaeta lycopersici, is an important soil-borne disease of tomato in many cultivated areas worldwide.
The use of resistant cultivars offers a suitable alternative to the conventional methods of disease control.
Little is known about the molecular and physiological processes underlying both tomato resistance and P. lycopersici pathogenicity during their interaction.
Changes in transcription in the roots of tomato challenged with a strain of P. lycopersici were analyzed by a cDNA-AFLP based approach.
After artificial infection of young tomato roots with a GUS transformed strain of P. lycopersici, typical symptoms of the disease were observed after 72–96 hours post-infection (hpi). The changes in transcriptional profiles were analyzed starting from 48 hpi to 27 days post-infection (dpi) because root colonization by P. lycopersici proceeds quite slowly.
More than 700 transcript-derived fragments (TDFs) showing polymorphism between infected and control roots have been isolated.
Of these fragments, about 40% was induced and 60% was repressed in infected tissue compared to the healthy plant.
The up-regulated fragments have been isolated from the gels, re-amplified and sequenced.
Comparison with genomic and EST databases was performed, with the aim of identifying and isolating one or more genes which might have a putative role in pathogenesis and/or resistance to corky root disease.
The use of resistant cultivars offers a suitable alternative to the conventional methods of disease control.
Little is known about the molecular and physiological processes underlying both tomato resistance and P. lycopersici pathogenicity during their interaction.
Changes in transcription in the roots of tomato challenged with a strain of P. lycopersici were analyzed by a cDNA-AFLP based approach.
After artificial infection of young tomato roots with a GUS transformed strain of P. lycopersici, typical symptoms of the disease were observed after 72–96 hours post-infection (hpi). The changes in transcriptional profiles were analyzed starting from 48 hpi to 27 days post-infection (dpi) because root colonization by P. lycopersici proceeds quite slowly.
More than 700 transcript-derived fragments (TDFs) showing polymorphism between infected and control roots have been isolated.
Of these fragments, about 40% was induced and 60% was repressed in infected tissue compared to the healthy plant.
The up-regulated fragments have been isolated from the gels, re-amplified and sequenced.
Comparison with genomic and EST databases was performed, with the aim of identifying and isolating one or more genes which might have a putative role in pathogenesis and/or resistance to corky root disease.
Publication
Authors
M. Aragona, A. Infantino
Keywords
corky root, Lycopersicon esculentum, disease resistance, pathogenesis
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