Articles
BIOLOGICAL CONTROL OF TOMATO FOOT AND ROOT ROT CAUSED BY FUSARIUM OXYSPORUM F.SP. RADICIS-LYCOPERSICI BY PSEUDOMONAS BACTERIA
Article number
808_50
Pages
317 – 320
Language
English
Abstract
Rhizobacteria are a natural and most suitable source for the isolation of potential microbiological control agents that can protect plants from soilborne pathogens and consequently improve crop quality and yield.
The beneficial effect of such bacteria on plant health depends in many cases on their ability to aggressively colonize the rhizosphere and compete with the indigenous, including pathogenic, microflora for nutrients and niches on the plant root.
Bacterial strains Pseudomonas chlororaphis PCL1391 and P. fluorescens WCS365 employ antibiosis and induced systemic resistance, respectively, to control tomato foot and root rot (TFRR) caused by phytopathogenic fungus Fusarium oxysporum f.sp. radicis-lycopersici (Forl). For the selection of biocontrol bacteria acting via the mechanism competition for nutrients and niches we have developed an enrichment method for enhanced tomato root tip colonizers, starting from a crude mixture of rhizobacteria coated on the seed, using a sterile quartz sand/plant nutrient solution gnotobiotic system.
As a result of this enrichment procedure, and subsequent tests on competitive tomato root tip colonization, the strongly competitive biocontrol strains P. fluorescens PCL1751 and P. putida PCL1760 were isolated.
Both strains effectively suppress TFRR under soil and hydroponic cultivation conditions.
The beneficial effect of such bacteria on plant health depends in many cases on their ability to aggressively colonize the rhizosphere and compete with the indigenous, including pathogenic, microflora for nutrients and niches on the plant root.
Bacterial strains Pseudomonas chlororaphis PCL1391 and P. fluorescens WCS365 employ antibiosis and induced systemic resistance, respectively, to control tomato foot and root rot (TFRR) caused by phytopathogenic fungus Fusarium oxysporum f.sp. radicis-lycopersici (Forl). For the selection of biocontrol bacteria acting via the mechanism competition for nutrients and niches we have developed an enrichment method for enhanced tomato root tip colonizers, starting from a crude mixture of rhizobacteria coated on the seed, using a sterile quartz sand/plant nutrient solution gnotobiotic system.
As a result of this enrichment procedure, and subsequent tests on competitive tomato root tip colonization, the strongly competitive biocontrol strains P. fluorescens PCL1751 and P. putida PCL1760 were isolated.
Both strains effectively suppress TFRR under soil and hydroponic cultivation conditions.
Publication
Authors
F. Kamilova, S. Validov, B. Lugtenberg
Keywords
biocontrol, antibiosis, induced systemic resistance, competition for nutrients and niches, Pseudomonas, tomato foot and root rot, Fusarium oxysporum f.sp. radicis-lycopersici
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