Articles
RAPID PROPAGATION FROM IN VITRO CULTURED SEEDLING SHOOT-TIPS OF CULINARY RHUBARB (RHEUM RHAPONTICUM L.)
Article number
923_12
Pages
91 – 96
Language
English
Abstract
Rhubarb (Rheum rhaponticum L.) has been cultivated as a vegetable crop in Europe, the US, Canada and Europe for more than a century.
As a health-enhanced plant it is less known in China because of lacking plant resources.
In the last few years culinary rhubarb was introduced to China, but shortage of planting materials has been a problem for commercial cultivation.
An efficient protocol for in vitro propagation of rhubarb from seedling shoot-tips is described.
Regeneration was achieved by culturing shoot-tips on MS medium supplemented with BA or KT in different combinations with NAA or IBA at concentrations of 0-3 mg/L. The highest multiplication efficacy was obtained on MS supplemented with 1.0 mg/L BA+1.0 mg/L NAA, regenerating 9.1 shoots from an explant.
Regenerated shoots rooted on 1/2 MS medium containing 0.5 mg/L NAA obtained the highest rooting percentage (92.6%). Rooted plantlets were successfully transferred in field with 90% of survival.
This study reports, for the first time, a method for in vitro plant propagation using seedling shoot tips of rhubarb.
It provides an efficient protocol for rapidly propagating planting materials for rhubarb cultivation.
As a health-enhanced plant it is less known in China because of lacking plant resources.
In the last few years culinary rhubarb was introduced to China, but shortage of planting materials has been a problem for commercial cultivation.
An efficient protocol for in vitro propagation of rhubarb from seedling shoot-tips is described.
Regeneration was achieved by culturing shoot-tips on MS medium supplemented with BA or KT in different combinations with NAA or IBA at concentrations of 0-3 mg/L. The highest multiplication efficacy was obtained on MS supplemented with 1.0 mg/L BA+1.0 mg/L NAA, regenerating 9.1 shoots from an explant.
Regenerated shoots rooted on 1/2 MS medium containing 0.5 mg/L NAA obtained the highest rooting percentage (92.6%). Rooted plantlets were successfully transferred in field with 90% of survival.
This study reports, for the first time, a method for in vitro plant propagation using seedling shoot tips of rhubarb.
It provides an efficient protocol for rapidly propagating planting materials for rhubarb cultivation.
Authors
Y. Zhao, Z. Cai, Y. Zhou, S. Chen
Keywords
rhubarb, propagation, in vitro culture, seedling shoot tip, rooting
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