ANTHER CULTURE OF GARDEN CHRYSANTHEMUM

The aim of this study was the production of haploid garden chrysanthemum plants for a breeding program by using anther culture.
Five garden chrysanthemum cultivars were used as anther donor plants.
The effects of this genotype and the addi-tion of different growth regulators in MS medium were analyzed.
Plants regenerated via anther culture were observed in morphology, cell physiology and cytogenetic.
A significant difference in the anther callusing and regeneration rate was found among the cultivars.
The most effective induction medium was basal MS plus 2.0 mg/L of BA + 1.0 mg/L of 2,4-D + 9% W/V sucrose, while the best differentiation medium was MS basal medium plus 2.0 mg/L of BA + 0.1 mg/L of NAA + 2%W/V sucrose.
Haploid (n=3x=27) plants were obtained; they appeared similar in appearance to diploid ones; however, they were somewhat smaller than their diploid counterpart.
Moreover, the chloroplast number in stomata guard cells of haploid was 12, half of the diploids.

XXVIII International Horticultural Congress on Science and Horticulture for People (IHC2010): International Symposium on Micro and Macro Technologies for Plant Propagation and Breeding in Horticulture

Y. Gao, B. Chen, J. Zhang

haploid, anther culture, ploidy, chloroplast number, chrysanthemum

https://doi.org/10.17660/ActaHortic.2011.923.14