Articles
HISTOLOGICAL INVESTIGATION ON GRAFT FORMATION IN PEAR/QUINCE (PYRUS COMMUNIS/CYDONIA OBLONGA) COMBINATIONS
Article number
923_43
Pages
291 – 298
Language
English
Abstract
It is known that success of grafting in higher plants depends on two essential factors: the physiological compatibility between the two graft partners, scion and root¬stock and the proper alignment of like tissues.
Vascular tissue formation is the last stage of successful grafting.
Formation of a strong union depends on differentiation and amount of new vascular elements formed after grafting.
Histological evaluation on graft sections gives us first information about compatibility or incompatibility of combinations in short time.
This research was aimed to determine the anatomical structure of graft union in some heterograft combinations of pear/quince.
The study was carried out in the University of Agricultural Sciences and Veterinary Medicine Iasi Experimental Farm during 2007-2009. In the study four scion cultivars and quince as rootstock were used.
Tissue samples were taken from graft unions one year after grafting and fixed in formalin/glacial acetic acid/alcohol solution.
Cambium activity, necrotic layers, cambial continuity and vascular tissues formation were analyzed.
It was observed from the anatomical structure of graft union area that new cambium, xylem and phloem tissues were formed and a longer time was needed for continuous cambial merging.
Some abnormalities were seen at the graft union area in some combinations and it was suggested that there could be a not very good compatibility for these graft combinations.
The applicability of this study could be the possibility to apply an early selection method that could predict the future of a determined combination long before the external symptoms can be observed.
Vascular tissue formation is the last stage of successful grafting.
Formation of a strong union depends on differentiation and amount of new vascular elements formed after grafting.
Histological evaluation on graft sections gives us first information about compatibility or incompatibility of combinations in short time.
This research was aimed to determine the anatomical structure of graft union in some heterograft combinations of pear/quince.
The study was carried out in the University of Agricultural Sciences and Veterinary Medicine Iasi Experimental Farm during 2007-2009. In the study four scion cultivars and quince as rootstock were used.
Tissue samples were taken from graft unions one year after grafting and fixed in formalin/glacial acetic acid/alcohol solution.
Cambium activity, necrotic layers, cambial continuity and vascular tissues formation were analyzed.
It was observed from the anatomical structure of graft union area that new cambium, xylem and phloem tissues were formed and a longer time was needed for continuous cambial merging.
Some abnormalities were seen at the graft union area in some combinations and it was suggested that there could be a not very good compatibility for these graft combinations.
The applicability of this study could be the possibility to apply an early selection method that could predict the future of a determined combination long before the external symptoms can be observed.
Authors
C. Zlati, G. Grădinariu, M. Istrate , L. Draghia
Keywords
grafting, Pyrus communis, Cydonia oblonga, compatibility, anatomical structure
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