Articles
SIMULTANEOUS PRODUCTION OF CYTOPLASMIC SUBSTITUTION LINE AND ALIEN MONOSOMIC ADDITION LINE THROUGH INTERSPECIFIC HYBRIDIZATION BETWEEN ALLIUM ROYLEI AND A. CEPA
Article number
969_5
Pages
65 – 71
Language
English
Abstract
Allium roylei (genomes RR, 2n=16) was employed as donor species for producing two different plant materials in order to explore its new possibilities in breeding purposes: 1) development of cytoplasmic substitution lines and 2) production of alien monosomic addition lines (AMALs) in A. cepa. The chromosomes of a single F1 plant between A. roylei (female) and shallot were doubled, and then backcrossing with shallot or bulb onion was performed to produce BC1 as allotriploids.
One allotriploid plant was used for backcrossing with bulb onion as seed parent (BC2: BR line) and selfing (BC1F2: SR line). Another plant was used for backcrossings with bulb onions as pollen parents (BC2: RB and SY lines). The BC2 showed 2n=16, 17, 18, 19, 22, 23, and 24. The BC2 plants with 2n=16 in RB line (68 plants) showed relatively low pollen fertility ranging from 0% to more than 10% in Yamaguchi, Japan.
In Kagawa and Hokkaido, most of the plants were completely pollen sterile, while the percentages of seed sets in BC2 were high enough to reproduce BC3 seeds.
The results revealed that the substitution of A. roylei cytoplasm would be useful for the development of a novel CMS line in Allium. Forty-eight BC2 and BC1F2 plants that have 2n=17 were used for the characterization of alien chromosome from A. roylei via chromosome specific markers (three isozymes and ten EST markers). In the AMALs, a complete set of eight monosomic lines (CC+nR, n=1~8) with different frequency of plants was obtained: CC+1R (one plant), CC+2R (one), CC+3R (two), CC+4R (one), CC+5R (two), CC+6R (one), CC+7R (two), and CC+8R (14). All the plants formed dormant bulbs in different sizes and colors.
These novel AMALs would be the potential materials not only for the breeding of A. cepa but also for the genetic studies of A. roylei.
One allotriploid plant was used for backcrossing with bulb onion as seed parent (BC2: BR line) and selfing (BC1F2: SR line). Another plant was used for backcrossings with bulb onions as pollen parents (BC2: RB and SY lines). The BC2 showed 2n=16, 17, 18, 19, 22, 23, and 24. The BC2 plants with 2n=16 in RB line (68 plants) showed relatively low pollen fertility ranging from 0% to more than 10% in Yamaguchi, Japan.
In Kagawa and Hokkaido, most of the plants were completely pollen sterile, while the percentages of seed sets in BC2 were high enough to reproduce BC3 seeds.
The results revealed that the substitution of A. roylei cytoplasm would be useful for the development of a novel CMS line in Allium. Forty-eight BC2 and BC1F2 plants that have 2n=17 were used for the characterization of alien chromosome from A. roylei via chromosome specific markers (three isozymes and ten EST markers). In the AMALs, a complete set of eight monosomic lines (CC+nR, n=1~8) with different frequency of plants was obtained: CC+1R (one plant), CC+2R (one), CC+3R (two), CC+4R (one), CC+5R (two), CC+6R (one), CC+7R (two), and CC+8R (14). All the plants formed dormant bulbs in different sizes and colors.
These novel AMALs would be the potential materials not only for the breeding of A. cepa but also for the genetic studies of A. roylei.
Publication
Authors
H.Q. Vu, T. Nakajima, Y. Yoshimatsu, M. Iwata, N. Yamauchi, M. Shigyo
Keywords
Allium roylei, shallot, bulb onion, alloplasmic male sterile, alien monosomic addition lines
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