Articles
IDENTIFICATION OF APPLE SPORT MUTANTS OF ‘ANTONOVKA’ BY MOLECULAR METHODS
Article number
976_40
Pages
299 – 303
Language
English
Abstract
Apple has high intraspecific polymorphism of some genome sequences that allow the development of effective methods for DNA-identification, in particular with SSR markers.
These methods enable significant distinction between species, cultivars, hybrids, but not between sport mutants.
However, sport mutants differing from initial cultivars in morphological traits have been described for some apple cultivars including the old Russian cultivar Antonovka. This investigation was carried out to study genetic diversity of sport mutants derived from the cultivar Antonovka and if possible, identify them by molecular methods.
Ten accessions of this cultivar that differ in morphological traits were used in this study.
They were collected from orchards located in different regions of Belarus.
SSR analysis was applied using 20 markers which were known to have a high level of polymorphism among apple cultivars.
The results obtained have shown that only one of ten accessions differed in the allele composition at the loci studied indicating its cross-pollination origin.
The other nine accessions did not differ in the allele composition for these 20 SSR loci therefore no effective identification of sport mutants could be provided by this approach.
No differences between these accessions were revealed by methods of ISSR analysis using 10 primers or RAPD analysis using 27 primers.
The S-SAP method, using 33 combinations of primers, one of which borders the sequence of a retrotransposon, was applied for detailed genetic analysis of Antonovka sport mutants.
Individual polymorphic fragments between accessions were revealed by this approach.
These methods enable significant distinction between species, cultivars, hybrids, but not between sport mutants.
However, sport mutants differing from initial cultivars in morphological traits have been described for some apple cultivars including the old Russian cultivar Antonovka. This investigation was carried out to study genetic diversity of sport mutants derived from the cultivar Antonovka and if possible, identify them by molecular methods.
Ten accessions of this cultivar that differ in morphological traits were used in this study.
They were collected from orchards located in different regions of Belarus.
SSR analysis was applied using 20 markers which were known to have a high level of polymorphism among apple cultivars.
The results obtained have shown that only one of ten accessions differed in the allele composition at the loci studied indicating its cross-pollination origin.
The other nine accessions did not differ in the allele composition for these 20 SSR loci therefore no effective identification of sport mutants could be provided by this approach.
No differences between these accessions were revealed by methods of ISSR analysis using 10 primers or RAPD analysis using 27 primers.
The S-SAP method, using 33 combinations of primers, one of which borders the sequence of a retrotransposon, was applied for detailed genetic analysis of Antonovka sport mutants.
Individual polymorphic fragments between accessions were revealed by this approach.
Authors
O.YU. Urbanovich, P.V. Kuzmitskaya, N.A. Kartel, Z.A. Kazlouskaya
Keywords
Malus × domestica, sequence-specific amplified polymorphism (S-SAP)
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