Articles
GENETIC MAPPING AND MARKER-ASSISTED SELECTION OF THE GENE CONFERRING SUSCEPTIBILITY TO ALTERNARIA BLOTCH CAUSED BY ALTERNARIA ALTERNATE APPLE PATHOTYPE IN APPLE
Article number
976_79
Pages
555 – 560
Language
English
Abstract
Alternaria blotch, caused by the Alternaria alternate apple pathotype, is an important fungal disease that adversely affects apple production.
A major gene Alt controls resistance and/or susceptibility to this disease, and the resistant genotype is indicated as alt/alt. In a progeny of 114 F1 individuals derived from reciprocal crosses between Starking Delicious (susceptible, Alt/alt) and Jonathan (resistant, alt/alt), Alt was mapped to the top of linkage group (LG) 11 by integrated mapping.
The nearest marker was DR033892, which is an expressed sequence tag (EST)-derived marker transferred from the linkage map of the powdery mildew resistance genes Pl2 and PlMIS located at the top of LG 11 (Gardiner et al., 2007; Chagné et al., 2008) using the comparative genomic approach.
DR033892 was converted to a usable marker by designing primers generating a unique PCR product of 429 bp from the Alt-coupled allele.
All of the eight susceptible cultivars tested inherited the Alt-coupled allele of DR033892, suggesting that DR033892 can be used in marker-assisted selection (MAS) with crosses between susceptible cultivars that generate a 429-bp fragment and resistant cultivars that do not generate the same 429-bp fragment.
A major gene Alt controls resistance and/or susceptibility to this disease, and the resistant genotype is indicated as alt/alt. In a progeny of 114 F1 individuals derived from reciprocal crosses between Starking Delicious (susceptible, Alt/alt) and Jonathan (resistant, alt/alt), Alt was mapped to the top of linkage group (LG) 11 by integrated mapping.
The nearest marker was DR033892, which is an expressed sequence tag (EST)-derived marker transferred from the linkage map of the powdery mildew resistance genes Pl2 and PlMIS located at the top of LG 11 (Gardiner et al., 2007; Chagné et al., 2008) using the comparative genomic approach.
DR033892 was converted to a usable marker by designing primers generating a unique PCR product of 429 bp from the Alt-coupled allele.
All of the eight susceptible cultivars tested inherited the Alt-coupled allele of DR033892, suggesting that DR033892 can be used in marker-assisted selection (MAS) with crosses between susceptible cultivars that generate a 429-bp fragment and resistant cultivars that do not generate the same 429-bp fragment.
Authors
S. Moriya, S. Terakami, H. Iwanami, T. Haji, K. Okada, T. Yamamoto, K. Abe
Keywords
expressed sequence tag, genome scanning approach, Malus × domestica Borkh., resistance breeding, simple sequence repeat
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