Articles
DETECTION OF PEAR S-ALLELES BY SETTING UP A REVISED IDENTIFICATION SYSTEM
Article number
976_46
Pages
339 – 343
Language
English
Abstract
Pear (Pyrus communis L.) cultivars are self-incompatible and their adequate fruit set depends on the presence of pollinizer trees with compatibility of bloom period and alleles.
This research was carried out in order to identify the self-incompatibility alleles in pear cultivars of the Iranian National Pear Collection.
Partial or complete sequences of more than 200 pear S-alleles have been obtained from NCBI and aligned by ClustalW2 in order to compare their conserved regions for categorization of the sequences and subsequent primer design.
All deposited S-alleles were separated based on the original species and sequence in 63 divergent sequences, and C2 and C3 conserved regions were used for FB-F/FB-R2 non-specific primer designing.
Following PCR amplification of S-alleles, DraI, EcoRI, MspI and HaeIII restriction maps of amplified regions were compared to expected maps from previously identified S-alleles.
The results showed homonym groups of alleles, also synonymous alleles such as S22 and S3 in the data bank.
Detection of S-alleles in pear cultivars demonstrated 21, 10.5, 13.1 and 7.8% of frequencies, respectively for S1, S2, S4 and S5 alleles.
Interestingly, the S42 rare allele from P. ussuriensis was detected in cultivar Konjuni originating from central areas of Iran.
This research was carried out in order to identify the self-incompatibility alleles in pear cultivars of the Iranian National Pear Collection.
Partial or complete sequences of more than 200 pear S-alleles have been obtained from NCBI and aligned by ClustalW2 in order to compare their conserved regions for categorization of the sequences and subsequent primer design.
All deposited S-alleles were separated based on the original species and sequence in 63 divergent sequences, and C2 and C3 conserved regions were used for FB-F/FB-R2 non-specific primer designing.
Following PCR amplification of S-alleles, DraI, EcoRI, MspI and HaeIII restriction maps of amplified regions were compared to expected maps from previously identified S-alleles.
The results showed homonym groups of alleles, also synonymous alleles such as S22 and S3 in the data bank.
Detection of S-alleles in pear cultivars demonstrated 21, 10.5, 13.1 and 7.8% of frequencies, respectively for S1, S2, S4 and S5 alleles.
Interestingly, the S42 rare allele from P. ussuriensis was detected in cultivar Konjuni originating from central areas of Iran.
Authors
F. Babaei, M. Khorramdel Azad, H. Abdollahi, S. Torabi, M. Aminafshar
Keywords
primer design, self-incompatibility, PCR-RFLP
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