Articles
APPLICATION OF SRAP MOLECULAR MARKERS FOR THE IDENTIFICATION OF ZOYSIAGRASS HYBRIDS
Article number
1035_25
Pages
223 – 232
Language
English
Abstract
Zoysiagrass is a popular warm season turfgrass throughout the world.
Two Zoysia F1 segregating populations of the reciprocal crosses of Z136 (cold tolerant) × Z039 (limited cold tolerance) were obtained in order to determine the genetic mechanism of and genetic markers for cold tolerance in zoysiagrass.
SRAP (Sequence-related Amplified Polymorphism) was used to determine true hybrids among 184 progeny of Z136 × Z039 and 103 progeny of Z039 × Z136. Two hundred SRAP primer combinations were tested to discriminate between Z136 and Z039. Twenty-six primer combinations revealed bands specific to Z039 but absent in Z136, and 35 primer combinations revealed the specific bands present in Z136 and absent in Z039. Eleven primer combinations with the specific bands of Z039 and other eleven primer combinations with the specific bands of Z136 were used to identify the true hybrids among the progeny.
The results showed that 168 progeny of Z136 × Z039 and 99 progeny of Z039 × Z136 showed bands specific to the male parent, which were identified to be true hybrids.
The remaining progeny were considered to be the result of self-pollination.
This has demonstrated that SRAP molecular marker technology could be used effectively to identify true hybrids for further study of cold tolerance in zoysiagrass.
Two Zoysia F1 segregating populations of the reciprocal crosses of Z136 (cold tolerant) × Z039 (limited cold tolerance) were obtained in order to determine the genetic mechanism of and genetic markers for cold tolerance in zoysiagrass.
SRAP (Sequence-related Amplified Polymorphism) was used to determine true hybrids among 184 progeny of Z136 × Z039 and 103 progeny of Z039 × Z136. Two hundred SRAP primer combinations were tested to discriminate between Z136 and Z039. Twenty-six primer combinations revealed bands specific to Z039 but absent in Z136, and 35 primer combinations revealed the specific bands present in Z136 and absent in Z039. Eleven primer combinations with the specific bands of Z039 and other eleven primer combinations with the specific bands of Z136 were used to identify the true hybrids among the progeny.
The results showed that 168 progeny of Z136 × Z039 and 99 progeny of Z039 × Z136 showed bands specific to the male parent, which were identified to be true hybrids.
The remaining progeny were considered to be the result of self-pollination.
This has demonstrated that SRAP molecular marker technology could be used effectively to identify true hybrids for further study of cold tolerance in zoysiagrass.
Authors
H.L. Guo, D.D. Xue, X. Chen, Y.Q. Zheng, Z.Y. Wang, J.X. Liu
Keywords
Zoysia, cross breeding, true hybrids, SRAP, identification
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