Articles
METABOLIC COLD ACCLIMATION OF ‘POLKA’ AND ‘HONEOYE’ STRAWBERRIES UNDER NATURAL FIELD CONDITIONS
Article number
1049_67
Pages
463 – 466
Language
English
Abstract
The winter hardiness of strawberry cultivars used in perennial production systems varies greatly.
Still, little information is available on how plant metabolism adapts to cold and freezing temperatures under natural temperature and light conditions.
In order to examine the hardening process of overwintering meristematic tissue in Fragaria × ananassa, crown samples of field-grown cultivars Polka and Honeoye were consecutively collected over a period of 15 weeks, i.e. from the end of the season (week 35/end August) until midwinter (week 50/December). Samples were subjected to qGC MS metabolite profiling to assess the reconfiguration of central metabolism, and characterize the regulation of selected compatible solutes.
Besides changes in amino acid patterns (glutamic acid, aspartic acid, and asparagine), monosaccharide levels (fructose) increased strongly in Honeoye (180 fold compared to start control) towards the end of the acclimation period.
In contrast, Polka showed a concentration peak (36-fold) in week 47 and a decline towards week 50. Also sucrose levels were steadily increased throughout the cold hardening period with averagely 6-fold higher levels in Honeoye compared to Polka, thus underscoring cultivar-dependent differences.
However, both cultivars showed a decline in sucrose levels after week 47. Particularly, the raffinose pathway was affected leading to strongly and transiently increased levels of the precursor galactinol (week 42/mid-October) and the trisaccharide raffinose (weeks 43-47/end October to mid-November). While galactinol biosynthesis was earlier induced in Polka (week 38) compared to Honeoye (week 39), subsequent raffinose production was delayed in Polka (week 47) compared to Honeoye (week 45). Major metabolic changes in both cultivars coincided with a decrease in day length below 14 h in mid-September, and a consistent drop below 10°C average day temperature by the end of September.
Still, little information is available on how plant metabolism adapts to cold and freezing temperatures under natural temperature and light conditions.
In order to examine the hardening process of overwintering meristematic tissue in Fragaria × ananassa, crown samples of field-grown cultivars Polka and Honeoye were consecutively collected over a period of 15 weeks, i.e. from the end of the season (week 35/end August) until midwinter (week 50/December). Samples were subjected to qGC MS metabolite profiling to assess the reconfiguration of central metabolism, and characterize the regulation of selected compatible solutes.
Besides changes in amino acid patterns (glutamic acid, aspartic acid, and asparagine), monosaccharide levels (fructose) increased strongly in Honeoye (180 fold compared to start control) towards the end of the acclimation period.
In contrast, Polka showed a concentration peak (36-fold) in week 47 and a decline towards week 50. Also sucrose levels were steadily increased throughout the cold hardening period with averagely 6-fold higher levels in Honeoye compared to Polka, thus underscoring cultivar-dependent differences.
However, both cultivars showed a decline in sucrose levels after week 47. Particularly, the raffinose pathway was affected leading to strongly and transiently increased levels of the precursor galactinol (week 42/mid-October) and the trisaccharide raffinose (weeks 43-47/end October to mid-November). While galactinol biosynthesis was earlier induced in Polka (week 38) compared to Honeoye (week 39), subsequent raffinose production was delayed in Polka (week 47) compared to Honeoye (week 45). Major metabolic changes in both cultivars coincided with a decrease in day length below 14 h in mid-September, and a consistent drop below 10°C average day temperature by the end of September.
Publication
Authors
J. Rohloff, P. Eidem, J. Davik, M. Alsheikh
Keywords
Fragaria × ananassa Duch., winter hardiness, metabolite profiling, quadrupole gas chromatography-mass spectrometry (qGC-MS), temperature, light
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