Articles
A MEMBRANE-BINDING CONSERVED MOTIF IN THE COAT PROTEIN OF PNMV SEEMS TO MEDIATE CHLOROPLAST TARGETING
Article number
1072_10
Pages
97 – 103
Language
English
Abstract
Replication of all positive-sense single-stranded RNA viruses occurs in specific structures in close association with cellular membranes.
Targeting of the viral replication complex (RC) to the site of replication is mediated by the interaction of viral-encoded proteins and host factors.
Electron microscope studies have shown that Poinsettia mosaic virus (PnMV, family Tymoviridae) infection is associated with the presence of vesicular structures in the chloroplasts, which indicates that the replication of PnMV might occur in association with chloroplast-derived membranes.
Using computer assisted homology search, we have identified that the coat protein (CP) of PnMV shows similarity to membrane bound proteins and contains a conserved amino acid sequence motif found in members of the Alb3/Oxa1/YidC protein family.
This protein family is involved in the insertion of proteins into intracellular membranes.
In this study we carried out localization studies combined with confocal laser microscopy to identify the cellular localization of the PnMV CP. Transient expression of red fluorescent protein (RFP)-tagged PnMV CP in Nicotiana benthamiana protoplast was shown to localize in the chloroplast.
Targeting of the viral replication complex (RC) to the site of replication is mediated by the interaction of viral-encoded proteins and host factors.
Electron microscope studies have shown that Poinsettia mosaic virus (PnMV, family Tymoviridae) infection is associated with the presence of vesicular structures in the chloroplasts, which indicates that the replication of PnMV might occur in association with chloroplast-derived membranes.
Using computer assisted homology search, we have identified that the coat protein (CP) of PnMV shows similarity to membrane bound proteins and contains a conserved amino acid sequence motif found in members of the Alb3/Oxa1/YidC protein family.
This protein family is involved in the insertion of proteins into intracellular membranes.
In this study we carried out localization studies combined with confocal laser microscopy to identify the cellular localization of the PnMV CP. Transient expression of red fluorescent protein (RFP)-tagged PnMV CP in Nicotiana benthamiana protoplast was shown to localize in the chloroplast.
Authors
C. Spetz, D.-R. Blystad
Keywords
Poinsettia mosaic virus, coat protein, chloroplast
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