Articles
SUPPORTIVE AND ANTAGONISTIC INTERACTIONS AMONG POSPIVIROIDS INFECTING SOLANACEOUS ORNAMENTALS
Article number
1072_7
Pages
71 – 77
Language
English
Abstract
In recent years, viroids have become an increasingly prominent issue for phytosanitary measures in the ornamental industry.
In particular, four species belonging to Pospiviroid genus (Potato spindle tuber viroid – PSTVd, Tomato apical stunt viroid – TASVd, Chrysanthemum stunt viroid – CSVd and Citrus exocortis viroid – CEVd) were found to infect Solanum jasminoides and other ornamentals in several European countries.
For this reason, studies were carried out to improve the understanding on how pospiviroids, mainly PSTVd, can be transmitted from infected to healthy plants.
In the frame of a research project funded by the Italian Ministry of Agriculture, transmission experiments consisting in infecting PSTVd-free S. jasminoides plants were performed and yielded unsuccessful results.
Subsequent diagnostic tests have revealed the presence of CEVd in all plants used for the infection with PSTVd.
To understand the real role of CEVd in the failure to transmit PSTVd on S. jasminoides, tomato was chosen as model plant and interactions between PSTVd, CEVd and TASVd were studied.
Pospiviroid isolates were obtained from natural single infected S. jasminoides plants.
Results of challenge tests showed that only TASVd was able to co-infect the already CEVd and PSTVd infected plants.
On the other hand, TASVd acted as an antagonistic viroid against PSTVd and CEVd challenge inoculation and, furthermore, CEVd was shown to protect from PSTVd infection.
These results do not fully elucidate on the natural mixed infection of PSTVd and CEVd that occur in S. jasminoides plants assayed during our surveys.
In contrast, they explain our failure to transmit PSTVd into already CEVd infected plants.
In particular, four species belonging to Pospiviroid genus (Potato spindle tuber viroid – PSTVd, Tomato apical stunt viroid – TASVd, Chrysanthemum stunt viroid – CSVd and Citrus exocortis viroid – CEVd) were found to infect Solanum jasminoides and other ornamentals in several European countries.
For this reason, studies were carried out to improve the understanding on how pospiviroids, mainly PSTVd, can be transmitted from infected to healthy plants.
In the frame of a research project funded by the Italian Ministry of Agriculture, transmission experiments consisting in infecting PSTVd-free S. jasminoides plants were performed and yielded unsuccessful results.
Subsequent diagnostic tests have revealed the presence of CEVd in all plants used for the infection with PSTVd.
To understand the real role of CEVd in the failure to transmit PSTVd on S. jasminoides, tomato was chosen as model plant and interactions between PSTVd, CEVd and TASVd were studied.
Pospiviroid isolates were obtained from natural single infected S. jasminoides plants.
Results of challenge tests showed that only TASVd was able to co-infect the already CEVd and PSTVd infected plants.
On the other hand, TASVd acted as an antagonistic viroid against PSTVd and CEVd challenge inoculation and, furthermore, CEVd was shown to protect from PSTVd infection.
These results do not fully elucidate on the natural mixed infection of PSTVd and CEVd that occur in S. jasminoides plants assayed during our surveys.
In contrast, they explain our failure to transmit PSTVd into already CEVd infected plants.
Authors
L. Tomassoli, D. Luison, M. Luigi, E. Costantini, P. Mangiaracina, F. Faggioli
Keywords
Solanum jasminoides, tomato, PSTVd, CEVd, TASVd, cross-protection
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