Articles
FIRST REPORT OF ALSTROMERIA VIRUS X FROM XEROCHRYSUM BRACTEATUM IN NORWAY
Article number
1072_16
Pages
135 – 140
Language
English
Abstract
A virus (isolate HelE91-1) was isolated from leaves of strawflower (Xerochrysum bracteatum, syn. Helichrysum bracteatum, syn. Bracteantha bracteata) with mottle symptoms in Norway.
Preliminary results from electron microscopy revealed a flexuous virus with a dominant particle length of ~500 nm, identifying it as a putative member of the genus Potexvirus. Molecular and biological methods were further used to determine the coat protein (CP) sequence and the host range of isolate HelE91-1, respectively. Potexvirus-specific degenerate primers were designed and used to amplify a ~300 nucleotide fragment belonging to the RNA-dependent RNA polymerase region of the viral genome by reverse-transcription PCR (RT-PCR). Based on the results from the sequence analysis, new primers were designed to amplify a fragment encompassing the CP gene.
Furthermore, isolate HelE91-1 was mechanically inoculated to different test plants and found to systemically infect thirteen plant species belonging to four families.
CP sequence comparisons, at amino acid and nucleotide level, as well as phylogenetic analysis showed that isolate HelE91-1 had considerable homology to the Japanese isolate of Alstromeria virus X (AlsVX) (80% nt-level, 91% aa-level) and the Finnish isolate, AlsVX-Hel (89% nt level, 98% aa-level).
Preliminary results from electron microscopy revealed a flexuous virus with a dominant particle length of ~500 nm, identifying it as a putative member of the genus Potexvirus. Molecular and biological methods were further used to determine the coat protein (CP) sequence and the host range of isolate HelE91-1, respectively. Potexvirus-specific degenerate primers were designed and used to amplify a ~300 nucleotide fragment belonging to the RNA-dependent RNA polymerase region of the viral genome by reverse-transcription PCR (RT-PCR). Based on the results from the sequence analysis, new primers were designed to amplify a fragment encompassing the CP gene.
Furthermore, isolate HelE91-1 was mechanically inoculated to different test plants and found to systemically infect thirteen plant species belonging to four families.
CP sequence comparisons, at amino acid and nucleotide level, as well as phylogenetic analysis showed that isolate HelE91-1 had considerable homology to the Japanese isolate of Alstromeria virus X (AlsVX) (80% nt-level, 91% aa-level) and the Finnish isolate, AlsVX-Hel (89% nt level, 98% aa-level).
Authors
M.W. Dees, C. Spetz, D.-R. Blystad
Keywords
plant virus, Potexvirus, strawflower
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