Articles
IDENTIFICATION AND MOLECULAR CHARACTERIZATION OF MULTIPLE PHYTOPLASMA INFECTION IN SPARTIUM JUNCEUM AND CYTISUS SCOPARIUS
Article number
1072_12
Pages
113 – 116
Language
English
Abstract
Two genera of the Fabaceae family showed phytoplasma symptoms in Germany and in Italy. Spartium junceum showing typical SpaWB symptoms was observed in the city of Ercolano (Campania region, Italy) and a similar symptomatology was observed in a group of shrubs of Cytisus scoparius growing in Dahlem botanical garden in Berlin (Germany). Symptomatic and asymptomatic samples were collected in both cases and analysed for phytoplasma presence by nested-PCR assays followed by RFLP analyses.
All symptomatic samples produced amplicons of the expected lengths and no product was amplified from asymptomatic plants.
Identification and classification of phytoplasmas allowed detection of Candidatus Phytoplasma spartii, subgroup 16SrX-D, and Ca. P. asteris, subgroup 16SrI-B, in both genera.
In some of the samples of C. scoparius, stolbur phytoplasma was also identified.
All symptomatic samples produced amplicons of the expected lengths and no product was amplified from asymptomatic plants.
Identification and classification of phytoplasmas allowed detection of Candidatus Phytoplasma spartii, subgroup 16SrX-D, and Ca. P. asteris, subgroup 16SrI-B, in both genera.
In some of the samples of C. scoparius, stolbur phytoplasma was also identified.
Authors
N. Contaldo, S. Paltrinieri, S. Ardizzi, A. Bertaccini, M.G. Bellardi, B. Duduk
Keywords
diseases, symptomatology, PCR, RFLP, molecular characterization
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