Articles
CHARACTERIZATION OF A NEW CARLAVIRUS FROM GAILLARDIA ARISTATA
Article number
1072_15
Pages
129 – 133
Language
English
Abstract
During investigation of Gaillardia aristata breeding material several plants reacted strongly (DAS-ELISA) with a commercially available Chrysanthemum virus B (CVB) antiserum.
In order to confirm the identity of the virus, a part of its replicase gene was sequenced, showing just 26% amino acid sequence identity to CVB. This prompted us to determine the entire genome of this virus isolate.
The complete genome was 8659 nt in length (excluding poly-A tail) and contained six open reading frames.
The genome organisation resembled that of typical carlaviruses.
The replicase (70%) and CP (71%) showed the highest aa sequence identities to Phlox virus S (PhVS), being well below the species demarcation threshold of 80%. The remaining ORFs (TGB1-3, NABP) also showed the highest aa sequence identities to PhVS, ranging from 59 (TGB3) to 77% (NABP). In addition, the CVB antiserum was tested with other Carlavirus isolates available at the DSMZ plant virus collection.
Besides CVB and the new Carlavirus from Gaillardia, it showed a strong cross-reaction (DAS-ELISA) with isolates of Kalanchoe latent virus, Potato virus S, Passiflora latent virus and Helenium virus S.
In order to confirm the identity of the virus, a part of its replicase gene was sequenced, showing just 26% amino acid sequence identity to CVB. This prompted us to determine the entire genome of this virus isolate.
The complete genome was 8659 nt in length (excluding poly-A tail) and contained six open reading frames.
The genome organisation resembled that of typical carlaviruses.
The replicase (70%) and CP (71%) showed the highest aa sequence identities to Phlox virus S (PhVS), being well below the species demarcation threshold of 80%. The remaining ORFs (TGB1-3, NABP) also showed the highest aa sequence identities to PhVS, ranging from 59 (TGB3) to 77% (NABP). In addition, the CVB antiserum was tested with other Carlavirus isolates available at the DSMZ plant virus collection.
Besides CVB and the new Carlavirus from Gaillardia, it showed a strong cross-reaction (DAS-ELISA) with isolates of Kalanchoe latent virus, Potato virus S, Passiflora latent virus and Helenium virus S.
Authors
W. Menzel, J. Hamacher, S. Winter
Keywords
Carlavirus, Gaillardia aristata, serological reaction, ELISA, Gaillardia latent virus, GLV, PV-0989
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