Articles
APPLICATION OF SEQUENCE-RELATED AMPLIFIED POLYMORPHISM (SRAP) ANALYSIS ON CULTIVAR IDENTIFICATION OF HERBACEOUS PEONY
Article number
1100_13
Pages
99 – 104
Language
English
Abstract
Sequence-related amplified polymorphism (SRAP) analysis was tried to apply
on the identification of 45 individuals of 26 cultivars of herbaceous peony (Paeonia
lactiflora Pall), which were conserved in three different gardens.
Selected five out of 25
primer pairs generated a total of 49 scorable PCR fragments, of which 42 bands (85.7%)
were polymorphic.
All 26 cultivars were distinguishable by these bands.
Some morphologically
confused individuals can be identified by this analysis.
Cluster analysis of
SRAP markers using UPGMA analysis revealed genetic relationships among the 26
cultivars.
The results indicated that SRAP markers are useful to identify germplasm
collection of herbaceous peony.
on the identification of 45 individuals of 26 cultivars of herbaceous peony (Paeonia
lactiflora Pall), which were conserved in three different gardens.
Selected five out of 25
primer pairs generated a total of 49 scorable PCR fragments, of which 42 bands (85.7%)
were polymorphic.
All 26 cultivars were distinguishable by these bands.
Some morphologically
confused individuals can be identified by this analysis.
Cluster analysis of
SRAP markers using UPGMA analysis revealed genetic relationships among the 26
cultivars.
The results indicated that SRAP markers are useful to identify germplasm
collection of herbaceous peony.
Authors
T. Handa, K. Shimada, I. Masuda, T. Horikoshi
Keywords
Paeonia lactiflora, historical collection, cultivar conservation, molecular marker, homonym, synonym
Online Articles (27)