Articles
IDENTIFICATION OF UNKNOWN ALMOND GENOTYPES BY SSR ANALYSIS
Article number
1100_23
Pages
149 – 153
Language
English
Abstract
The aim of this work was identification of almond genotypes in the old orchards
which are intended to be restored.
An analysis of microsatellite loci was used for genotypes
identification and for genetic diversity study within 23 analysed almond accessions.
12 genotypes works as reference samples from germplasm collection of cultivars
registered in the Czech Republic.
Rest of 11 genotypes were unknown genotypes from
the orchards around Hustopeče city (Czech Republic), whereas Hustopeče area is only
typical region for almond planting in Czech Republic.
These unknown genotypes were
collected from trees with highest morphological differences.
For analysis were used 6
SSR (Simple Sequence Repeats) markers derived from apricot genome.
An UPGMA
(Unweighted Pair Group Method with Arithmetic Mean) analysis divided analysed
almond genotypes into three major clusters. 4 ones from unknown genotypes had same
SSR profile as cultivar Zora, 4 ones shown similarity with Nikitskij cultivar and 3
ones from unknown genotypes were not similar with any cultivar from germplasm
collection.
which are intended to be restored.
An analysis of microsatellite loci was used for genotypes
identification and for genetic diversity study within 23 analysed almond accessions.
12 genotypes works as reference samples from germplasm collection of cultivars
registered in the Czech Republic.
Rest of 11 genotypes were unknown genotypes from
the orchards around Hustopeče city (Czech Republic), whereas Hustopeče area is only
typical region for almond planting in Czech Republic.
These unknown genotypes were
collected from trees with highest morphological differences.
For analysis were used 6
SSR (Simple Sequence Repeats) markers derived from apricot genome.
An UPGMA
(Unweighted Pair Group Method with Arithmetic Mean) analysis divided analysed
almond genotypes into three major clusters. 4 ones from unknown genotypes had same
SSR profile as cultivar Zora, 4 ones shown similarity with Nikitskij cultivar and 3
ones from unknown genotypes were not similar with any cultivar from germplasm
collection.
Authors
J. Cechová, M. Baránek, V. Holleinová
Keywords
Amygdalus communis L., microsatellites, cultivar identifications
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