Articles
CHARACTERISATION OF GENETIC DIVERSITY OF THE LITHUANIAN SOUR CHERRY (PRUNUS CERASUS L.) GENETIC RESOURCES USING MICROSATELLITE MARKERS
Article number
1100_14
Pages
105 – 108
Language
English
Abstract
Thirty seven sour cherry (Prunus cerasus L.) accessions were evaluated using
eight microsatellite (SSR) primer pairs in aim to characterise genetic diversity in the
sour cherry collection of Institute of Horticulture Lithuanian Research Centre for Agriculture
and Forestry (LRCAF IH). Seven to 20 alleles per primer pair were identified
in the multiplex analysis set-up.
The primer pair PCEGA34 was a most polymorphic
and was able to discriminate 33 among the 37 sour cherry cultivars.
A set of three
microsatellite markers that distinguish all sour cherry cultivars used in the study was
established.
Cluster analysis of the microsatellite marker data established genetic
relationship among the cultivars and distinguished cultivars with a different level of
adaptation to climate conditions.
eight microsatellite (SSR) primer pairs in aim to characterise genetic diversity in the
sour cherry collection of Institute of Horticulture Lithuanian Research Centre for Agriculture
and Forestry (LRCAF IH). Seven to 20 alleles per primer pair were identified
in the multiplex analysis set-up.
The primer pair PCEGA34 was a most polymorphic
and was able to discriminate 33 among the 37 sour cherry cultivars.
A set of three
microsatellite markers that distinguish all sour cherry cultivars used in the study was
established.
Cluster analysis of the microsatellite marker data established genetic
relationship among the cultivars and distinguished cultivars with a different level of
adaptation to climate conditions.
Authors
R. Rugienius, J.B. Frercks, B. Siksnianienė, I. Stepulaitienė, G. Stanienė, D. Baniulis, V. Stanys
Keywords
genetic variation, germplasm, microsatellite, heterozygosity, cultivars
Online Articles (27)