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DETECTION OF TULIP-BREAKING VIRUS IN TULIPS BY THE ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA)

Article number
110_10
Pages
89 – 90
Language
Abstract
As part of the quality improvement and inspection programm of The Netherlands Bulb Inspection Service, a test was needed for the serological diagnosis of viruses, including tulipbreaking virus (TBV) in tulips.

The enzyme-linked immunosorbent assay (ELISA) proved to be a sensitive test.

Special precautions are necessary for the preparation of a TBV antiserum such as serological purification of the TBV suspension by the complete removal of contaminations, i.e., lily symptomless virus (LSV) and fraction-1-proteins, with anti-LSV and anti-Fl sera.
During the growing season, TBV was detected in tulip leaves and bulbs by ELISA. During storage, TBV could be detected in bulbs from primarily infected tulips.

Publication
V International Symposium on Virus diseases of Ornamental Plants
Authors
A.R. van Schadewijk, J. Eggink
Keywords
Full text
https://doi.org/10.17660/ActaHortic.1980.110.10
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