Articles
HONEYSUCKLE LATENT VIRUS, A CARLAVIRUS INFECTING LONICERA PERICLYMENUM AND L. JAPONICA (CAPRIFOLIACEAE)
HLV was transmissible in the non-persistent manner by the aphid Hydaphis foeniculi, and by sap-inoculation to 8 of 22 species from 4 of 8 families.
It was best cultured in Nicotiana clevelandii and assayed in C. murale.
The virus is moderately stable in vitro; sap from N. clevelandii was infective after dilution to 1/1,000 but not 1/10,000, after 10 min at 80 but not 85°C, and after 4–8 days at 20°C or 32–64 days at 2°C. HLV has filamentous particles mostly c. 13 x 650 nm which sediment as a single component with a sedimentation coefficient of 157 S, and contain c. 5% single-stranded RNA of mol. wt 2.8 million and 95% protein of a single polypeptide of mol. wt 31,000.
These properties place HLV in the carlavirus group; it is serologically distantly related to red clover vein mosaic and poplar mosaic viruses, but is probably unrelated to 14 other members of the group.