Marker-assisted introgression of black-rot resistance gene Xca1bc into cauliflower through interspecific hybridization

Black rot is an important bacterial disease of Brassica vegetables throughout the world, caused by Xanthomonas campestris pv. campestris (Xcc). This disease takes a heavy toll of cole crops, causing yield losses of 10-50%. Since there is lack of black-rot resistance in Brassica oleracea (C genome), exploration of other Brassica species in the Uthant triangle is necessary, as they are supposed to be potential reservoirs of genes for black-rot disease resistance.
Resistance to black-rot disease in Brassica carinata NPC-9, governed by a single dominant gene, is flanked by markers At1g70610 and At1g71865 on linkage group B-7. To introgress this resistance into cauliflower, an interspecific hybrid and its backcross progeny (B₁) were generated between cauliflower LSQUOPusa SharadRSQUO and Ethiopian mustard NPC-9 using in-vitro embryo rescue techniques.
Hybridity confirmation of F₁ interspecific plants was carried out with a co-dominant simple sequence repeat marker (NI2-C01) and Brassica B and C genome-specific primers DB and DC. The F₁ interspecific plants showed resistance on artificial inoculation with Xcc race 1. Segregation distortion was observed in BC₁ generation for black-rot resistance.
Successful introgression of black-rot resistance in the interspecific BC₁ generation was confirmed by At1g70610 marker analysis.
This paper reports marker-assisted introgression of B genome-specific Xcc resistance from B. carinata to cauliflower through embryo rescue technique.

IV International Symposium on Molecular Markers in Horticulture

P. Kalia, B.B. Sharma, D. Singh

cole crop, Brassica oleracea, Ethiopian mustard, Brassica carinata, embryo rescue, Xanthomonas campestris pv. campestris

https://doi.org/10.17660/ActaHortic.2018.1203.8